HPLC Determination of SudanⅠ-Ⅳ in Food with Molecularly Imprinted Solid Phase Extraction
摘 要
以苏丹红Ⅰ为模板分子,甲基丙烯酸为功能单体合成了苏丹红分子印迹聚合物,作为固相萃取吸附剂,用于食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的测定。样品经乙腈提取,所得提取液分子印迹固相萃取柱,然后用乙醇-正己烷(1+1)溶液作洗脱剂将苏丹红洗下,收集洗出液供高效液相色谱测定。结果表明:聚合物对印迹分子具有很好的亲和性和特异选择性。方法的线性范围为0.5~15 mg·L-1,检出限(3S/N)为4 μg·kg-1。以辣椒粉和辣椒酱为基体,在3个浓度水平下做加标回收试验,回收率在70.3%~85.5%之间,测定值的相对标准偏差(n=5)低于6.0%。
Abstract
The molecularly imprinted polymer of Sudan red was synthesized with Sudan Ⅰ as template molecule and methacrylic acid as functional monomer, and it was used to extract Sudan Ⅰ-Ⅳ from food as adsorbent in solid phase extraction column. The sample was extracted with acetonitrile, and the extract obtained were purified on MIP-SPE column. Sudan Ⅰ-Ⅳ adsorbed on the imprinted polymer were eluted with ethanol-n-hexane (1+1) mixture, which were collected and used for HPLC determination. It was found that the imprinted polymer had good affinity and excellent selectivity for SudanⅠ-Ⅳ. Linearity range for the determination was found between 0.5-15 mg·L-1, and detection limit (3S/N) was 4 μg·kg-1. Using chili powder and chili sauce as matrixes, test for recovery was made at 3 concentration levels, values of recovery found were in the range of 70.3%-85.5% with RSD′s (n=5) less than 6.0%.
中图分类号 O657.7
所属栏目 工作简报
基金项目 广东高校优秀青年创新人才培育项目(209030)
收稿日期 2011/11/28
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备注徐小艳(1977-),女,湖南邵阳人,高级实验师,硕士,研究方向为食品安全检测。
引用该论文: XU Xiao-yan,DENG Shi-jun,SUN Yuan-ming. HPLC Determination of SudanⅠ-Ⅳ in Food with Molecularly Imprinted Solid Phase Extraction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(2): 153~155
徐小艳,邓世俊,孙远明. 分子印迹固相萃取-高效液相色谱法测定食品中苏丹红Ⅰ-Ⅳ[J]. 理化检验-化学分册, 2013, 49(2): 153~155
被引情况:
【1】张红园,林郑忠,吴泉书,林毅东,高治昊,黄志勇, "分子印迹-固相萃取-高效液相色谱法测定鱼肉中的孔雀石绿",理化检验-化学分册 51, 390-393(2015)
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参考文献
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【8】WANG Shuo, XU Zhi-xiang, FANG Guo-zhen, et al. Synthesis and characterization of a molecularly imprinted silica gel sorbent for the on-line determination of trace Sudan Ⅰ in chilli powder through high-performance liquid chromatography[J]. J Agric Food Chem, 2007,55(10):3869-3876.
【9】李保利,张敏,姜萍,等.表面接枝分子印迹聚合物微球的合成及评价[J].化学学报, 2007,65(10):955-961.
【2】XU Hai-yan, HEINZE T M, CHEN Si-wei, et al. An aerobic metabolism of 1-amino-2-naphthol-based azo dyes(Sudan dyes)by human intestinal microflora[J]. Applied and Environmental Microbiology, 2007,73(23):7759-7762.
【3】MASQUEE N, MARCEEK P M, BORRULL F. Molecularly imprinted polymers:new tailor-made materials for selective solid-phase extraction[J]. Trends in Analytical Chemistry, 2001,20(9):477-486.
【4】SHI Xi-zhi, WU Ai-bo, ZHENG Su-lian, et al. Molecularly imprinted polymer microspheres for solid-phase extraction of chloramphenicol residues in foods[J]. J Chromatogr B, 2007,850(1/2):24-30.
【5】ANDERSSON L. Molecular imprinting: developments and applications in the analytical chemistry field[J]. Journal of Chromatography B, 2000,745:3-13.
【6】FRANCESCO P, CARMELO G, FRANCESCA I, et al. Molecularly imprinted solid phase extraction for detection of Sudan Ⅰ in food matrices[J]. Food Chemistry, 2005,93(2):349-353.
【7】刘竟然,梁冰,李璐.苏丹红Ⅰ分子印迹聚合物制备及吸附性能研究[J].化学研究与应用, 2009,21(7):980-983.
【8】WANG Shuo, XU Zhi-xiang, FANG Guo-zhen, et al. Synthesis and characterization of a molecularly imprinted silica gel sorbent for the on-line determination of trace Sudan Ⅰ in chilli powder through high-performance liquid chromatography[J]. J Agric Food Chem, 2007,55(10):3869-3876.
【9】李保利,张敏,姜萍,等.表面接枝分子印迹聚合物微球的合成及评价[J].化学学报, 2007,65(10):955-961.
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