流动注射-分光光度法测定药物中盐酸吡硫醇含量
FI-Spectrophotometric Determination of Pyritinol Hydrochloride
摘 要
在pH 3.85乙酸盐缓冲溶液中,盐酸吡硫醇可将Fe(Ⅲ)还原为Fe(Ⅱ),邻菲啰啉能与生成的Fe(Ⅱ)显色,在波长508 nm处有最大吸收峰,据此提出了一种停留流动注射-分光光度法间接测定盐酸吡硫醇含量的方法。盐酸吡硫醇的质量浓度在8.00~500 mg·L-1范围内与其ΔA呈线性关系,方法的检出限(3s/k)为1.54 mg·L-1。方法用于盐酸吡硫醇药物中盐酸吡硫醇的测定,方法的回收率在99.6%~100%之间,测定值的相对标准偏差(n=6)小于2%。
分光光度法
盐酸吡硫醇
邻菲啰啉
Flow injection
Spectrophotometry
Pyritinol hydrochloride
o-Phenanthroline
Abstract
In an acetate buffer medium of pH 3.85, Fe(Ⅲ) was reduced by pyritinol hydrochloride to give Fe(Ⅱ), and a colored complex was formed by its reaction with o-phenanthroline, having its absorption maximum at the wavelength of 508 nm. Based on these facts, the determination of pyritinol hydrochloride by stopped flow injection (FI)-spectrophotometry was proposed. Linear relationship between values of ΔA and mass concentration of pyritinol hydrochloride was kept in the range of 8.00-500 mg·L-1, and detection limit (3s/k) of the method found was 1.54 mg·L-1. The proposed method was used in the determination of pyritinol hydrochloride in its drugs, and values of recovery found were in the range of 99.6%-100% with RSD (n=6) less than 2%.
中图分类号 O657.32
所属栏目 工作简报
基金项目 国家自然科学基金项目(30970696)
收稿日期 2011/12/6
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备注过治军(1979-),男,河南开封人,讲师,研究方向为光谱分析。
引用该论文: GUO Zhi-jun,LI Hua,LI Jing-yuan. FI-Spectrophotometric Determination of Pyritinol Hydrochloride[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(2): 202～204
过治军,李华,李景原. 流动注射-分光光度法测定药物中盐酸吡硫醇含量[J]. 理化检验－化学分册, 2013, 49(2): 202～204
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参考文献
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【5】BELAL F. Ion-pairing high-performance liquid chromatographic determination of pyridoxine and pyrithioxine in dosage forms［J］. Anal Lett, 1989,22(8):1897-1907.
【6】王海凤,王东凯,张蓓,等.高效液相色谱法测定盐酸吡硫醇粉针剂的含量［J］.药物分析杂志, 2007,27(4):564-565.
【7】EI-BARDICY M G, LOTFY H M, EI-TARRAS M F, et al. Stability indicating electrochemical methods for the determination of meclophenoxate hydrechloride and pyritinol dihydrochloride using ion-selective membrane electrodes［J］. Yakugaku Zasshi, 2007,127(1):201-208.
【8】占海红,张静,李晶,等.1,2-萘醌-4-磺酸钠测定盐酸吡硫醇的研究［J］.化学研究与应用, 2009,7(7):955-959.
【9】韩小燕,吴呈珂,冯素玲,等.光谱法研究Cu(Ⅱ)-核固红-盐酸吡硫醇体系及其分析应用［J］.河南师范大学学报:自然科学版, 2009,37(1):106-108.
【2】SHEHATA M A, EI-SAYED M A, EL-BARDICY M G, et al. Stability-indicating methods for determination of pyritinol dihydrochloride in the presence of its precursor and degradation product by derivative spectrophotometry［J］. J AOAC Int, 2005,88(1):80-86.
【3】SANE R T, SAMANT R S, NAYAK V G. Colorimetric determination of pyrithioxine from pharmaceutical preparations［J］. Indian J Pharm Sci, 1988,50(3):161-162.
【4】SASTRY C S P, MURALI KRISHNA D. Spectrophotometric determination of pyrithioxine in pharmaceutical formulations［J］. Anal Lett, 1995,28(7):1197-1207.
【5】BELAL F. Ion-pairing high-performance liquid chromatographic determination of pyridoxine and pyrithioxine in dosage forms［J］. Anal Lett, 1989,22(8):1897-1907.
【6】王海凤,王东凯,张蓓,等.高效液相色谱法测定盐酸吡硫醇粉针剂的含量［J］.药物分析杂志, 2007,27(4):564-565.
【7】EI-BARDICY M G, LOTFY H M, EI-TARRAS M F, et al. Stability indicating electrochemical methods for the determination of meclophenoxate hydrechloride and pyritinol dihydrochloride using ion-selective membrane electrodes［J］. Yakugaku Zasshi, 2007,127(1):201-208.
【8】占海红,张静,李晶,等.1,2-萘醌-4-磺酸钠测定盐酸吡硫醇的研究［J］.化学研究与应用, 2009,7(7):955-959.
【9】韩小燕,吴呈珂,冯素玲,等.光谱法研究Cu(Ⅱ)-核固红-盐酸吡硫醇体系及其分析应用［J］.河南师范大学学报:自然科学版, 2009,37(1):106-108.
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