二茂铁修饰碳糊电极示差脉冲伏安法测定槲皮素
Differential Pulse Voltammetric Determination of Quercetin with Ferrocene Modified Carbon Paste Electrode
摘 要
制备了二茂铁修饰碳糊电极,并采用循环伏安法研究了槲皮素在该修饰电极上的电化学行为。研究发现:在pH 6.2磷酸盐缓冲溶液中,修饰电极对槲皮素有良好的电催化作用,得到了一对氧化还原峰,其氧化反应是受吸附控制的两电子、两质子电极过程。并在此基础上提出了一种测定槲皮素的示差脉冲伏安法。试验结果表明:槲皮素的氧化峰电流与其浓度在8×10-4~2×10-6mol·L-1范围内呈线性关系,检出限(3S/N)为7×10-6mol·L-1。
槲皮素
碳糊电极
循环伏安法
示差脉冲伏安法
Ferrocene
Quercetin
Carbon paste electrode
Cyclic voltammetry
Differential pulse voltammetry
Abstract
Ferrocene modified carbon paste electrode was prepared, and the electrochemical behavior of quercetin of this modified electrode was studied by cyclic voltammetry. It was found that significant electro-catalytic action of the modified electrode was observed on the reaction of quercetin at the electrode, giving a pair of oxidation and reduction peak in PBS of pH 6.2. The oxidation of quercetin at the modified electrode is controlled mainly by adsorption in an electrode process involved with 2 electrons and 2 protons. Based on this fact, a method of differential pulse voltammetry was proposed for the determination of quercetin. Linear relationship between values of oxidation peak current and concentration of quercetin was obtained in the range from 8×10-4 to 2×10-6mol·L-1, with detection limit (3S/N) of 7×10-6mol·L-1.
中图分类号 O657.1
所属栏目 试验与研究
基金项目
收稿日期 2012/5/26
修改稿日期
网络出版日期
作者单位点击查看
联系人作者顾玲(anmslqwt@163.com)
备注顾玲(1962-),女,上海人,副教授,硕士,研究方向为电分析化学。
引用该论文: GU Ling,HE Ya-mei,ZHANG Miao,YUAN Miao-miao. Differential Pulse Voltammetric Determination of Quercetin with Ferrocene Modified Carbon Paste Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(3): 266～269
顾玲,贺亚梅,张苗,袁苗苗. 二茂铁修饰碳糊电极示差脉冲伏安法测定槲皮素[J]. 理化检验－化学分册, 2013, 49(3): 266～269
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参考文献
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【5】胡秋芬,杨光宇,黄章杰,等.微柱高效液相色谱法测定金银花中的多酚类物质［J］.分析化学, 2005,33(1):69-72.
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【9】邓湘辉,阚显文,尉艳,等.二茂铁/L-半胱氨酸修饰电极的电化学行为及电催化性能［J］.物理化学学报, 2005,21(12):1399-1402.
【10】白莉,郑志祥,高作宁.二茂铁修饰碳糊电极对抗坏血酸的电催化氧化及电分析方法研究［J］.化学传感器, 2007,27(3):53-57.
【11】吕紫玲,董绍俊.化学修饰电极的研究ⅩⅢ. 聚乙烯二茂铁(PVFc)薄膜电极和电催化效应［J］.物理化学学报, 1986,2(5):408-416.
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