提出了液相色谱法测定生物缓冲溶液中盐酸胍与氯离子含量。以Acclaim Trinity P1色谱柱(3.0 mm×100 mm,3 μm)为分离柱,乙腈和0.020 mol·L-1乙酸铵溶液按30比70混合(用乙酸调节pH至5.0)为流动相进行淋洗,用电雾式检测器检测,雾化器温度25 ℃,采样频率5 Hz。盐酸胍和氯离子的峰面积与质量浓度均在0.20~5.00 g·L-1范围内呈线性关系,方法检出限(3S/N)分别为0.005,0.001 g·L-1。采用标准加入法做回收试验,盐酸胍和氯离子的平均回收率分别为96.0%和92.5%,相对标准偏差(n=7)均小于1.0%。

LC was applied to the simultaneous determinations of guanidine hydrochloride and chloridion in bio-buffer. The sample were separated on Acclaim Trinity P1 column (3.0 mm×100 mm, 3 μm) with the mobile phase containing, acetonitrile and 0.020 mol·L-1 NH4OAc solution, mixed in the ratio of 30 to 70 (by volume) and adjusted to pH 5.0 with acetic acid, and the contents of guanidine hydrochloride and chloridion were determined by charged aerosol detector with atomizing temperature of 25 ℃ and sampling frequency of 5 Hz. Linear relationships between peak area and mass concentration for guanidine hydrochloride and chloridion were obtained in the same range of 0.20-5.00 g·L-1, and with their detection limits (3S/N) of 0.005 and 0.001 g·L-1 respectively. Values of average recovery found by standard addition method were 96.0% for guanidine hydrochloride and 92.5% for chloridion, with values of RSD′s (n=7) less than 1.0%.