高效液相色谱法测定聚L乳酸中乳酸单体含量
HPLC Determination of Lactic Acid Monomers in Poly L-Lactic Acid
摘 要
采用高效液相色谱法测定了药用辅料聚L乳酸中乳酸单体的残留量。采用正交试验优化了色谱的条件,以甲醇-0.1 mol·L磷酸二氢钾(5+95)混合液为流动相,流量为0.8 mL·min-1,柱温为40 ℃。乳酸的质量浓度在2.00~100 mg·L-1范围内与其峰高呈线性关系,方法的检出限(3S/N)为0.50 mg·L-1。对方法进行了回收率和精密度试验,样品的加标平均回收率为96%;测定值的相对标准偏差(n=6)为2.0%。
聚L乳酸
乳酸
HPLC
Poly L-lactic acid
Lactic acid
Abstract
HPLC was applied to the determination of residual amount of lactic acid monomer in the pharmaceutical adjuvant poly L-lactic acid. The optimized chromatographic conditions obtained by orthogonal test were as follow: a mixture of methanol and 0.1 mol·L-1 KH2PO4 solution, mixed in the ratio of 5 to 95 was used as mobile phase, with a flow-rate of 0.8 mL·min-1 and at the column temperature of 40 ℃. Linear relationship between values of peak height and mass concentration of lactic acid was kept in the range of 2.00-100 mg·L-1, with detection limit (3S/N) of 0.50 mg·L-1. Tests for recovery and precision were made, giving results of average recovery of 96% and RSD (n=6) of 2.0% respectively.
中图分类号 O652.63
所属栏目 工作简报
基金项目 安徽省十一五科技攻关重点项目(06013062A);安徽省教育厅自然科学重点项目(KJ2007135AZC)
收稿日期 2012/2/17
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备注王世亮(1956-),男,安徽无为人,教授,研究方向为化工新型材料,植入剂新药。
引用该论文: WANG Shi-liang,CHU Chen-ding,ZHU Yu-jun,BAO Shi-gen,HE Zhi-xia,XUAN Er-kang. HPLC Determination of Lactic Acid Monomers in Poly L-Lactic Acid[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(3): 326～328
王世亮,储成顶,朱玉俊,鲍时根,何志侠,宣尔康. 高效液相色谱法测定聚L乳酸中乳酸单体含量[J]. 理化检验－化学分册, 2013, 49(3): 326～328
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参考文献
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【4】陈琳,吴皓,王媚,等.中药泡制辅料醋中总酸和乳酸的含量测定［J］.中国中医药杂志, 2010,17(9):52-54.
【5】陆益民,宋光泉,闫杰,等.填充柱气相色谱法直接进样测定乳酸产品中乳酸含量［J］.食品与发酵工业杂志, 2007,33(9):154-155.
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【8】苏国岁,苏红军,何爱民.烟用香精中乳酸的快速气相色谱分析［J］.烟草科技/烟草检验, 2002,12(185):23-25.
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