HPLC Determination of 8 Anilines in Pressed Powder with Ultrasonic Assisted Extraction
摘 要
提出了高效液相色谱法测定粉饼中8种苯胺类物质(苯胺、对硝基苯胺、3-硝基苯胺、对甲苯胺、间氯苯胺、2,4-二硝基苯铵、2-氯-4-硝基苯胺、4-氯-2-甲基苯胺)含量的方法。样品中8种苯胺类物质用甲醇超声提取,离心分离后,所得提取液过0.45 μm有机滤膜。以SinoChrom ODS-BP色谱柱(4.6 mm×200 mm,5 μm)为分离柱,不同比例配成的甲醇和水混合溶液为流动相梯度洗脱,用紫外-可见光检测器于波长254 nm处检测。方法的检出限(3S/N)在0.01~0.07 μg·g-1之间,相对标准偏差(n=10)均小于1%。以空白粉饼样品为基体,用标准加入法测定方法的回收率,结果在87.5%~118%之间。
Abstract
Contents of 8 anilines (i.e., aniline, p-nitroaniline, 3-nitroaniline, p-toluidine, m-chloroaniline, 2,4-dinitroaniline, 2-chloro-4-nitroaniline and 4-chloro-2-methyl aniline) in pressed powder were determined by HPLC. The 8 anilines in the sample were extracted ultrasonically with methanol. After centrifugation, the extract was filtered on 0.45 μm organic filtering membrane. SinoChrom ODS-BP (4.6 mm×200 mm, 5 μm) chromatographic column was used as stationary phase, and solutions of methanol mixed with water in different ratios were used as mobile phase in the gradient elution. The eluate from the column was determined with UV-Vis detector at the wavelength of 254 nm. Detection limits (3S/N) of the method were found in the range of 0.01-0.07 μg·g-1, and RSD′s (n=10) found were less than 1%. Using blank sample of pressed powder as matrixes, values of recovery found by standard addition method were in the range of 87.5%-118%.
中图分类号 O652.63
所属栏目 工作简报
基金项目 浙江省自然科学基金(Y13B050017)
收稿日期 2012/7/22
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联系人作者卢珩俊(yzs@glut.edu.cn)
备注卢珩俊(1986-),男,浙江金华人,硕士研究生,主要研究方向为色谱分析与分离技术。
引用该论文: LU Heng-jun,CHEN Mei-lan,WU Hong-wei,JIANG Zhe-yan,YI Zhong-sheng. HPLC Determination of 8 Anilines in Pressed Powder with Ultrasonic Assisted Extraction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(7): 794~796
卢珩俊,陈梅兰,吴宏炜,姜浙燕,易忠胜. 超声辅助提取-高效液相色谱法测定粉饼中8种苯胺类物质的含量[J]. 理化检验-化学分册, 2013, 49(7): 794~796
被引情况:
【1】朱 颖,刘 沛,秦迪岚,宋冰冰,罗岳平,毕军平,朱日龙, "液液萃取-高效液相色谱法测定水中的苯胺和联苯胺",理化检验-化学分册 52, 840-842(2016)
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