镉离子对三种黄酮小分子与蛋白质相互作用的生物无机化学效应
Bioinorganic Effect of Cadmium Ion on the Interaction Between Micromolecules of 3 Flavonoids and Protein
摘 要
用荧光光谱法及紫外分光光度法研究了镉离子(Cd2+)对3种黄酮小分子槲皮素(Que)、金丝桃苷(Hyp)及芦丁(Rut)与牛血清白蛋白(BSA)相互作用的生物无机化学效应。对比了有无Cd2+存在下,3种黄酮与血清蛋白的结合常数、结合位点数、作用力及蛋白质构象等参数,结果表明:Cd2+与Hyp、Rut的结合部位位于其糖苷基团上,Cd2+在3种黄酮与BSA分子之间起“离子架桥”作用并直接参与了黄酮与BSA之间的结合,使两者更有利于插入蛋白质的疏水腔内部;而Que母核C环3位的羟基却不利于此“离子架桥”作用。“离子架桥”是Cd2+对黄酮小分子与BSA相互作用过程的主要参与机制。
牛血清白蛋白
镉离子
相互作用
Flavonoids molecules
Bovine serum albumin
Cadmium ion
Interaction
Abstract
The bioinorganic effect of cadmium ion (Cd2+) on the interaction between micromolecules of 3 flavonids ［i.e., quercetin (Que), hyperin (Hyp) and rutin (Rut)］ and bovine serum albumin (BSA) was studied by fluorospectrometry and UV-spectrophotometry. The parameters including the binding constants, number of binding sites, binding force and protein conformation in the absence and presence of Cd2+ were studied comparatively. It was found that the binding sites between Cd2+ and Hyp (or Rut) were located at its glucosyl group, and the Cd2+ was found to take part directly in the binding of the 2 flavonoids with BSA by acting as an “ion bridge”, thus making it favorable for both of the 2 flavonoids to insert into the hydrophobic cavity of the protein. But-off group sited at 3 of the carbon ring of mother nucleus of Que was found not favorable for such “ion bridge” action. It was proved that the “ion bridge” was the main participation mechanism for Cd2+ in the course of interaction between the micromolecules of the flavonoids and BSA.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 广西自然基金(21167006)
收稿日期 2012/10/4
修改稿日期
网络出版日期
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备注陶慧林(1961-),女,广西贺州人,副教授,研究方向为光谱分析。
引用该论文: TAO Hui-lin,XU Ming-ze,LI Shu-huai,LIU Shuai-tao,YI Zhong-sheng. Bioinorganic Effect of Cadmium Ion on the Interaction Between Micromolecules of 3 Flavonoids and Protein[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(10): 1149～1154
陶慧林,徐铭泽,黎舒怀,刘帅涛,易忠胜. 镉离子对三种黄酮小分子与蛋白质相互作用的生物无机化学效应[J]. 理化检验－化学分册, 2013, 49(10): 1149～1154
被引情况:
【1】陈毅挺,李艳霞,黄露,庄婷娟,林棋, "维生素K3的解离常数测定及其与牛血清白蛋白的相互作用",理化检验－化学分册 52, 61-65(2016)
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参考文献
【1】李继强,陈锡永.环境镉污染及其对人群健康影响的研究［J］.中国公共卫生, 2001,17(3):196-198.
【2】李文智.镉——危及人体健康的有毒元素［J］.中国环境卫生, 2006,9(1):18-22.
【3】谢佩意,胡万达.镉的致癌作用［J］.铁道劳动安全卫生与环保, 1989(2):57-60.
【4】李新枝,蔡佩玲,牟林春.镉致癌分子机制研究进展［J］.四川解剖学杂志, 2006,14(2):35-38.
【5】蒋新宇,李文秀,陈景文.锌离子存在下槲皮素、杨梅素与牛血清蛋白的结合［J］.无机化学学报, 2008,24(10):1588-1595.
【6】李满秀,窦艳枝.金丝桃苷与牛血清白蛋白的相互作用研究［J］.分析试验室, 2011,30(5):46-49.
【7】李丽萍,杨雪滢,单香丽,等.芦丁与牛血清蛋白在不同条件下的相互作用研究［J］.云南大学学报:自然科学版, 2012,34(1):72-83.
【8】LAKOWICZ J R, MASTERS B R. Principles of fluorescence spectroscopy［J］. Journal of Biomedical Optics, 2008,13:029901-029902.
【9】杨频,高飞,生物无机化学原理［M］.北京:科学出版社, 2002.
【10】张朝红,臧树良,耿兵,等.紫外和圆二色光谱法研究丁基锡化合物与牛血清白蛋白的相互作用［J］.分析科学学报, 2005,21(2):179-181.
【11】XIA Jiu-lin, DUBIN P L, KIM Y. Complex formation between poly (oxyethylene) and sodium dodecyl sulfate micelles: light scattering, electrophoresis, and dialysis equilibrium studies［J］. The Journal of Physical Chemistry, 1992,96(16):6805-6811.
【12】吴根华,汪婕,陈金龙,等.荧光光谱法研究锌(Ⅱ)存在下诺氟沙星与牛血清白蛋白的结合作用［J］.光谱学与光谱分析, 2008,28(4):913-916.
【13】SHEN L L, PERNET A G. Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA［J］. Proceedings of the National Academy of Sciences, 1985,82(2):307-311.
【14】ROSS P D, SUBRAMANIAN S. Thermodynamics of protein association reactions: forces contributing to stability［J］. Biochemistry, 1981,20(11):3096-3102.
【15】FRSTER T. Intermolecular energy transference and fluorescence［J］. Ann Physik, 1948,2:55-75.
【16】LIANG Hong, OUYANG Di, HU Xu-ying, et al. Structural studies on metal-serum albumin. III. slow conformational transition of HSA and BSA induced by Ni2+ ion［J］. Huaxue xuebao, 1998,56(7):662-667.
【17】黄宝圣.镉的生物毒性及其防治策略［J］.生物学通报, 2006,40(11):26-28.
【2】李文智.镉——危及人体健康的有毒元素［J］.中国环境卫生, 2006,9(1):18-22.
【3】谢佩意,胡万达.镉的致癌作用［J］.铁道劳动安全卫生与环保, 1989(2):57-60.
【4】李新枝,蔡佩玲,牟林春.镉致癌分子机制研究进展［J］.四川解剖学杂志, 2006,14(2):35-38.
【5】蒋新宇,李文秀,陈景文.锌离子存在下槲皮素、杨梅素与牛血清蛋白的结合［J］.无机化学学报, 2008,24(10):1588-1595.
【6】李满秀,窦艳枝.金丝桃苷与牛血清白蛋白的相互作用研究［J］.分析试验室, 2011,30(5):46-49.
【7】李丽萍,杨雪滢,单香丽,等.芦丁与牛血清蛋白在不同条件下的相互作用研究［J］.云南大学学报:自然科学版, 2012,34(1):72-83.
【8】LAKOWICZ J R, MASTERS B R. Principles of fluorescence spectroscopy［J］. Journal of Biomedical Optics, 2008,13:029901-029902.
【9】杨频,高飞,生物无机化学原理［M］.北京:科学出版社, 2002.
【10】张朝红,臧树良,耿兵,等.紫外和圆二色光谱法研究丁基锡化合物与牛血清白蛋白的相互作用［J］.分析科学学报, 2005,21(2):179-181.
【11】XIA Jiu-lin, DUBIN P L, KIM Y. Complex formation between poly (oxyethylene) and sodium dodecyl sulfate micelles: light scattering, electrophoresis, and dialysis equilibrium studies［J］. The Journal of Physical Chemistry, 1992,96(16):6805-6811.
【12】吴根华,汪婕,陈金龙,等.荧光光谱法研究锌(Ⅱ)存在下诺氟沙星与牛血清白蛋白的结合作用［J］.光谱学与光谱分析, 2008,28(4):913-916.
【13】SHEN L L, PERNET A G. Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA［J］. Proceedings of the National Academy of Sciences, 1985,82(2):307-311.
【14】ROSS P D, SUBRAMANIAN S. Thermodynamics of protein association reactions: forces contributing to stability［J］. Biochemistry, 1981,20(11):3096-3102.
【15】FRSTER T. Intermolecular energy transference and fluorescence［J］. Ann Physik, 1948,2:55-75.
【16】LIANG Hong, OUYANG Di, HU Xu-ying, et al. Structural studies on metal-serum albumin. III. slow conformational transition of HSA and BSA induced by Ni2+ ion［J］. Huaxue xuebao, 1998,56(7):662-667.
【17】黄宝圣.镉的生物毒性及其防治策略［J］.生物学通报, 2006,40(11):26-28.
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