纳米金标记抗体分光光度法测定尿液中盐酸克伦特罗
Spectrophotometric Determination of Clenbuterol Hydrochloride in Urine with Nano-Au Labelled Antibody
摘 要
金溶胶颗粒表面吸附盐酸克伦特罗单克隆抗体,可制得最大吸收波长在528 nm的纳米金标记抗体,此抗体用于分光光度法测定尿样中盐酸克伦特罗的含量。尿液中存在的盐酸克伦特罗(抗原)与纳米金颗粒吸附的抗体发生凝聚反应,可形成最大吸收波长在534 nm的抗原抗体复合物。盐酸克伦特罗的质量浓度在50 μg·L-1以内与其吸光度呈线性关系,方法的检出限(3S/N)为0.1 μg·L-1。采用此法测定了人尿液中盐酸克仑特罗的含量,所得测定值与国家标准方法中气相色谱-质谱法的测定结果相符。
盐酸克仑特罗
尿样
纳米金
抗体
Spectrophotometry
Clenbuterol hydrochloride
Urine sample
Nano-gold
Antibody
Abstract
Nano-Au labelled antibody (having its absorption maximum at the wavelength of 528 nm) was prepared by adsorption of monoclonal antibody of clenbuterol hydrochloride on the surface of nano-gold particles, and was used for spectrophotometric determination of clenbuterol hydrochloride in urine. When clenbuterol hydrochloride (the antigen) was present in the urine sample, immunoagglutination between antigen and the antibody adsorbed on the nano-Au particles occured forming a compound complex with its absorption maximum shifted to 534 nm. Linear relationship between values of absorbance and mass concentration of clenbuterol hydrochloride was obtained in the range within 50 μg·L-1, with detection limit (3S/N) of 0.1 μg·L-1. The proposed method was applied to the analysis of a human urine sample, giving result of content of clenbuterol hydrochloride in consistency with the result found by the GC-MS method given in GB standard.
中图分类号 O657.3
所属栏目 工作简报
基金项目 辽宁省质量技术监督局科技计划项目(2011104)
收稿日期 2012/10/20
修改稿日期
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备注王楠(1981-),女,辽宁沈阳人,助理研究员,硕士,主要研究方向为光谱分析。
引用该论文: WANG Nan,ZHOU Yu,DONG Zai-zheng. Spectrophotometric Determination of Clenbuterol Hydrochloride in Urine with Nano-Au Labelled Antibody[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(10): 1215～1218
王楠,周宇,董再蒸. 纳米金标记抗体分光光度法测定尿液中盐酸克伦特罗[J]. 理化检验－化学分册, 2013, 49(10): 1215～1218
被引情况:
【1】宋兴良,梁恕坤,王爱香,李云, "共振散射光谱法测定北虫草中的腺苷",理化检验－化学分册 52, 141-146(2016)
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参考文献
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【13】ZHANG Xiao-fang, ZHAO Hong, XUE Ying, et al. Colorimetric sensing of clenbuterol using gold nanoparticles in the presence of melamine［J］. Biosens Bioelectron, 2012,34:112-117.
【14】孟文瑛,郭志谋,沈伟健,等.一种新型固相萃取柱结合超高效液相色谱-串联质谱法选择性富集测定猪肉中的盐酸克伦特罗［J］.色谱, 2012,30(2):141-145.
【15】刘见,免疫金试纸法快速检测盐酸克伦特罗［J］.上海交通大学学报, 2004,22(4):350-354.
【16】王楠,徐淑坤,周宇.用葡萄糖制备纳米金及其与牛血清白蛋白偶联的研究［J］.分析科学学报, 2007,23(2):189-192.
【17】NY/QY 421-2003动物尿液中盐酸克伦特罗残留检测气相色谱-质谱法［S］.
【2】王选年,康晓迪,潘耀谦,等.盐酸克伦特罗的残留及检测［J］.动物医学进展, 2002,23(3):41-46.
【3】吴维明,蔡强,陈裕泉.纳米金在生物检测中的应用［J］.国外医学生物医学工程分册, 2003,26(5):193-197.
【4】FRENS G. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions［J］. Nature Phys Sci, 1973,241(5):20-22.
【5】BOGATYREV V A, DYKMAN L A, KRASONOV Y M, et al. Differential light scattering spectroscopy for studying biospecific assembling of gold nanoparticles with protein or oligonucleotide probes［J］. Colloid J, 2002,64(6):671-680.
【6】BADIA A, GAO W, SINGH S, et al. Structure and chain dynamics of alkanethiol-capped gold colloids［J］. Langmuir, 1996,12(5):1262-1269.
【7】DAI X H, TAN Y W, XU J. Formation of gold nanoparticles in the presence of o-anisidine and the dependence of the structure of poly(o-anisidine) on synthetic conditions［J］. Langmuir, 2002,18(23):9010-9016.
【8】兰新哲,金志浩,赵西成,等.PVP保护还原法制备纳米金溶胶［J］.稀有金属材料与工程, 2003,32(1):50-53.
【9】陈文星,吴雯,陈海相,等.丝素蛋白原位还原制备纳米贵金属胶体及表征［J］.中国科学B辑, 2003,33(3):185-191.
【10】LEE K M, PARK S T, LEE D J. Nanogold synthesis by inert gas condensation for immuno-chemistry probes［J］. J Alloys and Comp, 2005,390(2):297-300.
【11】MANDAL S, PHADTARE S, SASTRY M. Interfacing biology with nanoparticles［J］. Curr Appl Phys, 2005,5(2):118-127.
【12】王楠,徐淑坤,王文星.纳米金生物探针的应用新进展［J］.化学进展, 2007,19(2/3):408-413.
【13】ZHANG Xiao-fang, ZHAO Hong, XUE Ying, et al. Colorimetric sensing of clenbuterol using gold nanoparticles in the presence of melamine［J］. Biosens Bioelectron, 2012,34:112-117.
【14】孟文瑛,郭志谋,沈伟健,等.一种新型固相萃取柱结合超高效液相色谱-串联质谱法选择性富集测定猪肉中的盐酸克伦特罗［J］.色谱, 2012,30(2):141-145.
【15】刘见,免疫金试纸法快速检测盐酸克伦特罗［J］.上海交通大学学报, 2004,22(4):350-354.
【16】王楠,徐淑坤,周宇.用葡萄糖制备纳米金及其与牛血清白蛋白偶联的研究［J］.分析科学学报, 2007,23(2):189-192.
【17】NY/QY 421-2003动物尿液中盐酸克伦特罗残留检测气相色谱-质谱法［S］.
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