气相色谱-质谱法和高效液相色谱-串联飞行时间质谱法分析爬山虎吸盘分泌物
GC-MS and HPLC/Q-TOFMS Analysis of Sucker Secretion of Boston Ivy
摘 要
爬山虎吸盘分泌物样品用甲醇-氯仿(2+1)混合液提取并离心分离,取上清液用于分析。气相色谱-质谱法鉴定得到15种相对分子质量小于450且较易挥发的有机化合物,其中主要成分有谷甾醇、甲基-α-D-吡喃型葡萄糖苷、2,4-双-(1,1-二甲基乙基)苯酚、豆甾醇和棕榈酸等。从高效液相色谱-飞行时间质谱法所得质谱图,确认了19种较难挥发的有机化合物的经验分子式及其相对分子质量(Mr在200~920之间);而且在这些碳氢化合物中,12种化合物同时含氧及氮,3种化合物同时含氧、氮及硫。据此推断此类化合物易于产生氢键,而氢键的相互作用与爬山虎的攀爬功能存在一定关联。
高效液相色谱-串联飞行时间质谱法
爬山虎
吸盘分泌物
GC-MS
HPLC/Q-TOFMS
Boston ivy
Sucker secretion
Abstract
Sample of sucker secretion of Boston ivy was extracted with a mixture of methanol and chloroform (2+1), and after centrifugation, the supernatant was taken for analysis. Fifteen volatile organic compounds with Mr less than 450, including the main components of sitosterol, methyl-α-D-glucopyranoside, 2,4-bis-(1,1-dimethylethyl)-phenol, stigmasterol and palmitic acid, were identified by GC-MS; and for 19 hardly volatile organic compounds with Mr between 220 and 920, their empirical formulae and Mr, were confirmed by HPLC/Q-TOFMS. It was found that 12 of the 19 compounds were carbohydrates containing both N and O, and 3 of them containing also S in addition to N and O. It was deduced that these compounds were easy to produce hydrogen bonds, and that the interaction of hydrogen bonds was one of the factors related to the climbing property of the Boston ivy.
中图分类号 O657.63
所属栏目 工作简报
基金项目 辽宁省自然科学基金(201202100)
收稿日期 2012/9/19
修改稿日期
网络出版日期
作者单位点击查看
联系人作者田福林(fltian@sina.com)
备注田福林(1980-),男,山东淄博人,博士,主要从事分析化学研究工作。
引用该论文: TIAN Fu-lin,LI Hong,LIU Cheng-yan,WANG Zhi-jia,CHENG Guang-rong,ZHAO Hai-bo. GC-MS and HPLC/Q-TOFMS Analysis of Sucker Secretion of Boston Ivy[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(10): 1219～1222
田福林,李红,刘成雁,王志嘉,程光荣,赵海波. 气相色谱-质谱法和高效液相色谱-串联飞行时间质谱法分析爬山虎吸盘分泌物[J]. 理化检验－化学分册, 2013, 49(10): 1219～1222
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】何天贤,杨文伍,邓文礼.具有超级粘附作用的藤本植物——爬山虎的最新研究结果及研究进展评论［J］.自然科学进展, 2008,18(11):1220-1225.
【2】江仲春.爬山虎及川鄂爬山虎吸盘壁面附着机制的形态研究［J］.南京农业大学学报, 1994,17(4):27-31.
【3】ENDRESS A, THOMSON W. Adhesion of the Boston ivy tendril［J］. Can J Bot, 1977,55:918-924.
【4】ZHANG Ming-jun, LIU Mao-zi, PREST H, et al. Nanoparticles secreted from ivy rootlets for surface climbing［J］. Nano Letters, 2008,8(5):1277-1280.
【5】AUTUMN K, SITTI M, LIANG Y A, et al. Evidence for van der Waals adhesion in gecko setae［J］. Proc Natl Acad Sci U S A, 2002,99(19):12252-12256.
【6】PEATTIE A M, FULL R J. Phylogenetic analysis of the scaling of wet and dry biological fibrillar adhesives［J］. Proc Natl Acad Sci U S A, 2007,104(47):18595-18600.
【7】STEINBRECHER T, DANNINGER E, HARDER D, et al. Quantifying the attachment strength of climbing plants: a new approach［J］. Acta Biomater, 2010,6(4):1497-1504.
【8】HE Tian-xian, ZHANG Li, DENG Wen-li. Biological adhesion of Parthenocissus tricuspidata［J］. Arch Biol Sci, 2011,63(2):393-398.
【9】MELZER B, STEINBRECHER T, SEIDEL R, et al. The attachment strategy of English ivy: a complex mechanism acting on several hierarchical levels［J］. J R Soc Interface, 2010,7(50):1383-1389.
【10】XIA Li-jin, LENAGHAN S, ZHANG Ming-jun, et al. Characterization of English ivy (Hedera helix) adhesion force and imaging using atomic force microscopy［J］. Journal of Nanoparticle Research, 2011,13(3):1029-1037.
【11】LENAGHAN S C, ZHANG Ming-jun. Real-time observation of the secretion of a nanocomposite adhesive from English ivy (Hedera helix)［J］. Plant Science, 2011,183:206-211.
【12】LI Quan-shui, XIA Li-jin, ZHANG Zhi-li, et al. Ultraviolet extinction and visible transparency by ivy nanoparticles［J］. Nanoscale Res Lett, 2010,5(9):1487-1491.
【13】LENAGHAN S C, XIA L, HENSON W R, et al. Isolation and characterization of nanoparticles from English ivy (Hedera Helix)［J］. 27th Army Science Conference, 2010.
【14】VO T D T, HIMMELSBACH M, HAUNSCHMIDT M, et al. Improved analysis of melamine-formaldehyde resins by capillary zone electrophoresis-mass spectrometry using ion-trap and quadrupole-time-of flight mass spectrometers［J］. Journal of Chromatography A, 2008,1213:83-87.
【2】江仲春.爬山虎及川鄂爬山虎吸盘壁面附着机制的形态研究［J］.南京农业大学学报, 1994,17(4):27-31.
【3】ENDRESS A, THOMSON W. Adhesion of the Boston ivy tendril［J］. Can J Bot, 1977,55:918-924.
【4】ZHANG Ming-jun, LIU Mao-zi, PREST H, et al. Nanoparticles secreted from ivy rootlets for surface climbing［J］. Nano Letters, 2008,8(5):1277-1280.
【5】AUTUMN K, SITTI M, LIANG Y A, et al. Evidence for van der Waals adhesion in gecko setae［J］. Proc Natl Acad Sci U S A, 2002,99(19):12252-12256.
【6】PEATTIE A M, FULL R J. Phylogenetic analysis of the scaling of wet and dry biological fibrillar adhesives［J］. Proc Natl Acad Sci U S A, 2007,104(47):18595-18600.
【7】STEINBRECHER T, DANNINGER E, HARDER D, et al. Quantifying the attachment strength of climbing plants: a new approach［J］. Acta Biomater, 2010,6(4):1497-1504.
【8】HE Tian-xian, ZHANG Li, DENG Wen-li. Biological adhesion of Parthenocissus tricuspidata［J］. Arch Biol Sci, 2011,63(2):393-398.
【9】MELZER B, STEINBRECHER T, SEIDEL R, et al. The attachment strategy of English ivy: a complex mechanism acting on several hierarchical levels［J］. J R Soc Interface, 2010,7(50):1383-1389.
【10】XIA Li-jin, LENAGHAN S, ZHANG Ming-jun, et al. Characterization of English ivy (Hedera helix) adhesion force and imaging using atomic force microscopy［J］. Journal of Nanoparticle Research, 2011,13(3):1029-1037.
【11】LENAGHAN S C, ZHANG Ming-jun. Real-time observation of the secretion of a nanocomposite adhesive from English ivy (Hedera helix)［J］. Plant Science, 2011,183:206-211.
【12】LI Quan-shui, XIA Li-jin, ZHANG Zhi-li, et al. Ultraviolet extinction and visible transparency by ivy nanoparticles［J］. Nanoscale Res Lett, 2010,5(9):1487-1491.
【13】LENAGHAN S C, XIA L, HENSON W R, et al. Isolation and characterization of nanoparticles from English ivy (Hedera Helix)［J］. 27th Army Science Conference, 2010.
【14】VO T D T, HIMMELSBACH M, HAUNSCHMIDT M, et al. Improved analysis of melamine-formaldehyde resins by capillary zone electrophoresis-mass spectrometry using ion-trap and quadrupole-time-of flight mass spectrometers［J］. Journal of Chromatography A, 2008,1213:83-87.
相关信息
