分光光度法快速测定6种氨基甲酸酯类农药残留量
Rapid Spectrophotometric Determination of Residual Amounts of 6 Aminoformate Pesticides
摘 要
基于酶抑制法,提出了分光光度法测定6种氨基甲酸酯类农药残留量的方法。采用正交试验优化了获得乙酰胆碱酯酶最佳活性的条件:① 酶与底物的浓度比为6比1;② 显色剂用量为0.4 mg;③ 缓冲溶液的pH为8.5;④ 反应温度为40 ℃。在优化的试验条件下,6种氨基甲酸酯类农药的质量浓度在一定的范围内与抑制率I呈线性关系。方法用于果蔬样品分析,并在此基础上进行加标回收试验,回收率在82.0%~109%之间,测定值的相对标准偏差(n=6)在2.4%~3.6%之间。
氨基甲酸酯类农药
分光光度法
Acetylcholinesterase
Aminoformate pesticides
Spectrophotometry
Abstract
Based on enzyme inhibition, a method of spectrophotometric determination of residual amounts of 6 aminoformate pesticides was proposed. By orthogonal test, the optimum conditions to obtain favourable acetylcholinesterase activity were shown as follow: ① ratio of concentration of enzyme to substrate: 6∶1; ② amount of chromogenic agent: 0.4 mg; ③ pH value of buffer solution: 8.5; ④ temperature of reaction: 40 ℃. Linear relationships between values of inhibition rate I and mass concentration of the 6 aminoformate pesticides were kept in the definite ranges. Samples of fruits and vegetables were analyzed by the proposed method and test for recovery was made by standard addition method, giving values of recovery in the range from 82.0% to 109%, with RSD′s (n=6) in the range from 2.4% to 3.6%.
中图分类号 O657.32
所属栏目 工作简报
基金项目 国家质检总局科技计划项目(2012QK139)
收稿日期 2013/4/27
修改稿日期
网络出版日期
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备注周芳(1981-),女,黑龙江哈尔滨人,工程师,主要研究方向为天然产物研究与开发、食品营养与检测。
引用该论文: ZHOU Fang,ZHAO Xin,YANG Guang. Rapid Spectrophotometric Determination of Residual Amounts of 6 Aminoformate Pesticides[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(11): 1335～1338
周芳,赵鑫,杨光. 分光光度法快速测定6种氨基甲酸酯类农药残留量[J]. 理化检验－化学分册, 2013, 49(11): 1335～1338
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参考文献
【1】韦友欢,黄秋婵,谢燕青,等.农药残留对人体健康的危害效应及毒理机制［J］.广西民族师范学院学报, 2010,27(3):9-12.
【2】刘岩,马育松.固相萃取技术在农药残留分析中的应用［J］.河北大学学报:自然科学版, 2005,25(1):109-115.
【3】李英,周艳明,牛森.蔬菜、水果中氨基甲酸酯类农药多残留分析方法的研究［J］.现代科学仪器, 2005(6):68-70.
【4】GONCALVES C, ALPENDURADA M F. Solid-phase microextraction gas chromatography (tandem)-mass spectrometry as a tool for pesticide residue analysis in water samples at high sensitivity and selectivity with confirmation capabilities［J］. Journal of Chromatography A, 2004,1026(1/2):239-250.
【5】佟玲,李重九.含硫蔬菜中50种农药多残留的气相色谱-串联质谱检测技术研究［J］.分析测试学报, 2008,27(9):930-935.
【6】ZROSTLKOV J, HAJSLOV J, CAJKA T. Evaluation of two-dimensional gas chromatography time of flight-mass spectrometry for the determination of multile pesticide residues in fruit［J］. Journal of Chromatography A, 2003,1019(1/2):173-186.
【7】余孝颖.氨基甲酸酯类农药对不同生物来源的胆碱酯酶选择性抑制的研究［J］.环境科学, 2003,17(4):41-43.
【8】XU Song-che, WU An-bo, CHEN He-dong, et al. Production of a novel recombinant drosophila melanogaster acetylcholinestase for detection of organophosphate and carbamate insecticide residues［J］. Biomol Eng, 2007,24(2):253-261.
【2】刘岩,马育松.固相萃取技术在农药残留分析中的应用［J］.河北大学学报:自然科学版, 2005,25(1):109-115.
【3】李英,周艳明,牛森.蔬菜、水果中氨基甲酸酯类农药多残留分析方法的研究［J］.现代科学仪器, 2005(6):68-70.
【4】GONCALVES C, ALPENDURADA M F. Solid-phase microextraction gas chromatography (tandem)-mass spectrometry as a tool for pesticide residue analysis in water samples at high sensitivity and selectivity with confirmation capabilities［J］. Journal of Chromatography A, 2004,1026(1/2):239-250.
【5】佟玲,李重九.含硫蔬菜中50种农药多残留的气相色谱-串联质谱检测技术研究［J］.分析测试学报, 2008,27(9):930-935.
【6】ZROSTLKOV J, HAJSLOV J, CAJKA T. Evaluation of two-dimensional gas chromatography time of flight-mass spectrometry for the determination of multile pesticide residues in fruit［J］. Journal of Chromatography A, 2003,1019(1/2):173-186.
【7】余孝颖.氨基甲酸酯类农药对不同生物来源的胆碱酯酶选择性抑制的研究［J］.环境科学, 2003,17(4):41-43.
【8】XU Song-che, WU An-bo, CHEN He-dong, et al. Production of a novel recombinant drosophila melanogaster acetylcholinestase for detection of organophosphate and carbamate insecticide residues［J］. Biomol Eng, 2007,24(2):253-261.
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