Chronoamperometric Determination of Luteolin with Copper Nanocluster/Graphene Modified Glassy Carbon Electrode
摘 要
制备了铜纳米簇/石墨烯修饰玻碳电极(记为Cu/GP/GCE),并采用透射电子显微镜、扫描电子显微镜、交流阻抗法和循环伏安法对修饰电极的结构和性能进行表征。结果表明:该修饰电极对木犀草素的氧化具有协同催化作用,据此提出了计时电流法测定木犀草素的方法。在pH 4.0的磷酸盐缓冲溶液中,外加电位为0.8 V时,木犀草素的浓度在7.0×10-8~3.0×10-6mol·L-1范围内与其峰电流呈线性关系,检出限(3S/N)为3×10-8mol·L-1。该修饰电极用于菊花乙酸乙酯的提取物中木犀草素含量的测定,测定值与高效液相色谱法测定值相符,相对标准偏差(n=5)小于6.0%。
Abstract
The copper nanocluster/graphene modified glassy carbon electrode (abbreviated Cu/GP/GCE) was prepared, and the structure and property of the modified electrode were characterized by TEM, SEM, AC impedance and cyclic voltammetry. It was found that the sensor had synergistic electrocatalytic activity to the oxidation of luteolin. Based on this fact, a method of chronoamperometry for the determination of luteolin was proposed. Linear relationship between values of peak current and concentration of luteolin measured at the potential of 0.8 V in PBS of pH 4.0 was kept in the range of 7.0×10-8-3.0×10-6mol·L-1, with value of detection limit (3S/N) of 3 ×10-8mol·L-1. The modified electrode was used in the determination of luteolin in the ethyl acetate extract of chrysanthemum, giving results in consistency with those obtained by HPLC. Values of RSD (n=5) were found less than 6.0%.
中图分类号 O657.1
所属栏目 工作简报
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收稿日期 2012/9/27
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备注杨铁金(1963-),男,黑龙江绥化人,教授,硕士,主要研究方向为电化学分析和环境化学。
引用该论文: YANG Tie-jin,DU Jiang,SUN Hong-li,FU Fei. Chronoamperometric Determination of Luteolin with Copper Nanocluster/Graphene Modified Glassy Carbon Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(11): 1355~1359
杨铁金,杜江,孙洪力,富菲. 铜纳米簇/石墨烯修饰玻碳电极电流-时间法测定木犀草素[J]. 理化检验-化学分册, 2013, 49(11): 1355~1359
被引情况:
【1】周君,李侠,刁国旺, "还原氧化石墨烯-杯<参考文献原文>磺酸盐复合材料的制备及其电化学检测吡虫啉的应用",理化检验-化学分册 52, 278-283(2016)
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参考文献
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【2】GLORIA G, CARLOS G B. The flavonoids luteolin and quercetagetin inhibit lipoteichoic acid actions on H9c2 cardiomyocytes[J]. International Immunopharmacology, 2010,10:1003-1009.
【3】CAI Xue-ting, YE Ting-mei, LIU Chao, et al. Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells[J]. Toxicology in Vito, 2011,7(25):1385-1391.
【4】KAZUKI K, MARI U, HIROAKI Y, et al. Bioavailable flavonoids to suppress the formation of 8-OHdG in HepG2 cells[J]. Arch Biochem Biophys, 2006,455:197-203.
【5】SPCIL Z, SEIFRTOVA M, OPLETALB L, et al. Rapid qualitative and quantitative ultra high performance liquid chromatography method for simultaneous analysis of twenty nine common phenolic compounds of various structures[J]. Talanta, 2010,5(80):1970-1979.
【6】GOTTI R. Capillary electrophoresis of phytochemical substances in herbal drugs and medicinal plants[J]. Journal of Pharmaceutical and Biomedical Analysis, 2011,4(55):775-801.
【7】BARANOWSKA I, RAROG D. Application of derivative spectrophotometry to determination of flavonoid mixtures[J]. Talanta, 2001,55:209-212.
【8】LIU Chun-sheng, SONG Yun-shan, ZHANG Ke-jian, et al. Gas chromatographic/mass spectrometric profiling of luteolin and its metabolites in rat urine and bile[J]. Journal of Pharmaceutical and Biomedical Analysis, 1995,13:1409-1414.
【9】POTTS J R, DREYER D R, BIELAWSKI C W. Gaphene-based polymer nanocomposites[J]. Polymer, 2011,52:5-25.
【10】STANKOVICH S, DIKIN D A, DOMMETT G H B, et al. Graphene-based composite materials[J]. Nature, 2006,442:282-286.
【11】LI Jing, GUO Shao-jun, ZHAI Yue-ming, et al. High-sensitivity determination of lead and cadmium based on the Nafion-graphene composite film[J]. Anal Chim Acta, 2009,649:196-201.
【12】LI Jing, GUO Shao-jun, ZHAI Yue-ming, et al. Nafion-graphene nanocomposite film as enhanced sensing platform for ultrasensitive determination of cadmium[J]. Electrochem Commun, 2009,11:1085-1088.
【13】MOORE R B, WILKERSON J E, MARTIN C R. High-performance liquid chromatographic studies of the ion-exchange selectivity of Nafion[J]. Anal Chem, 1984,56:2572-2575.
【14】HUMMERS W, OFFEMAN R. Graphite oxide (GO) was prepared using the well-known Hummers method described by Hummers[J]. Am Chem Soc, 1958,80:1339-1339.
【15】STAKOVICH S, DIKIN D A, PINER R D, et al. Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide[J]. Carbon, 2007,45(7):1558-1565.
【16】CAO Liu-you, DIAO Peng, ZHU Tao, et al. Uniform electrochemical deposition of copper onto self-assembled gold nanoparticles[J]. Phys Chem B, 2004,108:3535-3539.
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