纳米金颗粒检测蔬菜中重金属铅的研究
Determination of Heavy Metal Pb in Vegetables with Gold Nanoparticles
摘 要
氯金酸经硼氢化钠还原成纳米金颗粒,取适量纳米金颗粒加入于氯金酸-碳酸钾混合溶液中,再加入浸食子酸(GA)-氯化钠混合溶液,使反应制得没食子酸功能化的纳米金颗粒(GA-AuNP′s)。当GA-AuNP′s与痕量铅(Ⅱ)反应时,由于颗粒表面的没食子酸与铅(Ⅱ)生成络合物,导致其局域表面等离子体共振(LSPR)吸收峰发生红移。铅(Ⅱ)浓度的对数与红移的波长(λ)在2.7×10-8~5.1×10-6mol·L-1范围内呈线性关系,其检出限(3S/N)达1×10-9mol·L-1。据此提出用GA-AuNP′s分光光度法测定蔬菜中痕量铅的方法。应用此方法测定了茄子中痕量铅,并用标准加入法做回收试验,测得回收率在93.1%~111%之间,测定值的相对标准偏差(n=6)均小于2%。
没食子酸
铅(Ⅱ)
蔬菜
分光光度法
Gold nanoparticles
Gallic acid
Pb(Ⅱ)
Vegetables
Spectrophotometry
Abstract
Aurichlorohydric acid, H［AuCl4］ was reduced by sodium borohydride to give gold nanoparticles (AuNP′s). An appropriate amount of the AuNP′s was added to a mixed solution of H［AuCl4］-K2CO3 and reacted with mixed solution of gallic acid (GA)-NaCl to give AuNP′s functionalized with gallic acid (abbrv. as GA-AuNP′s). It was shown that GA on the surface of AuNP′s can react with Pb(Ⅱ) ion to form a complex, leading to bathochromic shift of absorption maximum of localized surface plasma resonance (LSPR), and that linear relationship between values of lgcPb(Ⅱ) and magnitude of the bathochromic shift (λ) was kept in the range of 2.7×10-8 to 5.1×10-6mol·L-1 of Pb(Ⅱ), with detection limit (3S/N) of 1×10-9mol·L-1. Based on these facts, by using GA-AuNP′s, a spectrophotometric method for determination of traces of Pb(Ⅱ) in vegetables was proposed. An eggplant sample was analyzed by this method, and recovery was tested by standard addition method, giving results of recovery in the range of 93.1% to 111% with values of RSD′s (n=6) less than 2%.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 江苏省“青蓝工程”、江苏省高校科研成果产业化项目(JHB2012-67);江苏经贸职业技术学院重大课题(JMZ1201210)资助
收稿日期 2013/6/24
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备注蒋彩云(1976-),女,江苏南京人,讲师,博士,主要从事纳米材料在水平及环境方面的应用研究。
引用该论文: JIANG Cai-yun,MA Mei-hua,YU Fang,TAO En-jin,YANG Ai-ping,LI Xiao-hua. Determination of Heavy Metal Pb in Vegetables with Gold Nanoparticles[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(12): 1410～1413
蒋彩云,马美华,余芳,陶恩锦,杨爱萍,李小华. 纳米金颗粒检测蔬菜中重金属铅的研究[J]. 理化检验－化学分册, 2013, 49(12): 1410～1413
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【6】马美华,李小华,杨爱萍,等.催化动力学光度法测定对蔬菜中痕量铅的测定［J］.江苏农业科学, 2012,40(11):325-328．
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【9】ABOU-HASSAN A, SANDRE, NEVEU S, et al. Synthesis of goethite by separation of the nucleation and growth processes of ferrihydrite nanoparticles using microfluidics［J］. Angew Chem Int Ed, 2009,48:2342-2345．
【10】SCHMITTEL M, LIN Heng-wei. Quadruple-channel sensing: a molecular sensor with a single type of receptor site for selective and quantitative multi-ion analysis［J］. Angew Chem Int Ed, 2007,46:893-896．
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【12】YANTASEE W, LIN Yue-he, HONGSIRIKARN K. Electrochemical sensors for the detection of lead and other toxic heavy metals: the next generation of personal exposure biomonitors［J］. Environ Health, 2007,115:1683-1690．
【13】FIUZA S M, GOMES C, TEIXEIRA L J, et al. Phenolic acid derivatives with potential anticancer properties-a structure-activity relationship study. Part 1: methyl, propyl and octyl esters of caffeic and gallic acids［J］. Bioorg Med Chem, 2004,12(13):3581-3589．
【14】MCDONALD M, MILA I, SCALBERT A. Precipitation of metal Ions by plant polyphenols: ptimal conditions and origin of precipitation［J］. J Agric Food Chem, 1996,44(2):599-606．
【15】STRAWN D G, SPARKS D L. Effects of soil organic matter on the kinetics and mechanisms of Pb(Ⅱ) sorption and desorption in soil［J］. Soil Sci Soc Am J, 2000,64:144-156．
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