用线性扫描极谱法研究别嘌呤醇的电化学行为。在pH 4.48的乙酸-乙酸钠缓冲溶液中,别嘌呤醇于-1.534 V(vs.SCE)处产生一灵敏的还原波,其一次微分线性扫描峰电流与别嘌呤醇质量浓度在1.0~100.0 mg·L-1范围内呈线性关系,检出限(3S/N)为0.7 mg·L-1。对100.0 mg·L-1别嘌呤醇溶液进行6次平行试验,相对标准偏差(n=6)为0.89%。方法用于片剂中别嘌呤醇含量的测定,测定值与标示值相符。加入3个浓度水平的标准溶液做回收试验,测得平均回收率为99.2%。

It was found by linear scanning polarography, that in an HOAc-NaOAc buffer solution of pH 4.48 a sensitive reduction wave of allopurinol was observed at the potential of -1.534 V (vs.SCE). Linear relationship between values of the 1st derivative of peak current at -1.534 V and mass concentration of allopurinol was obtained in the range of 1.0-100.0 mg·L-1 with detection limit (3S/N) of 0.7 mg·L-1. Precision was tested with standard solution of allopurinol of 100.0 mg·L-1 for 6 determinations, value of RSD found was 0.89%. The proposed method was used in the determination of content of allopurinol in its tablets, giving results in consistency with the labelled value. Recovery was tested by addition of standard solution at 3 concentration levels, giving value of average recovery of 99.2%.