手性固定相-高效液相色谱法分离噁唑菌酮对映异构体
Resolution of Enantiomers of Famoxadone by HPLC with Chiral Stationary Phase
摘 要
采用Kromasil celluCoat手性色谱柱(4.6 mm×250 mm,5 μm)作为分离柱,用高效液相色谱法对噁唑菌酮对映异构体进行了拆分。在优化的试验条件下,正己烷-乙醇(70+30)混合液为流动相,流量为1.0 mL·min-1,柱温为25 ℃,噁唑菌酮对映异构体在10 min内成功分离。
高效液相色谱法
手性固定相
对映异构体
Famoxadone
HPLC
Chiral stationary phase
Enantiomers
Abstract
A method of HPLC was proposed for the resolution of enantiomers of famoxadone using Kromasil cellucoat chiral column (4.6 mm×250 mm, 5 μm) as separation column. A mixture of n-hexane and ethanol (70+30) was used as mobile phase with a flow-rate of 1.0 mL·min-1. Column temperature of 25 ℃ was chosen. Under the optimized conditions, enantiomers of famoxadone were successfully separated within 10 min.
中图分类号 O652.63
所属栏目 工作简报
基金项目
收稿日期 2012/11/27
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联系人作者陈建波(cjb0123@126.com)
备注陈建波(1982-),男,重庆垫江人,中级农艺师,硕士,主要从事农药分析及方法研究。
引用该论文: CHEN Jian-bo,MAO Jian-wei,WU Ai-juan. Resolution of Enantiomers of Famoxadone by HPLC with Chiral Stationary Phase[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(12): 1483～1485
陈建波,毛健伟,吴爱娟. 手性固定相-高效液相色谱法分离噁唑菌酮对映异构体[J]. 理化检验－化学分册, 2013, 49(12): 1483～1485
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参考文献
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【11】沈报春,袁建勇.二茂铁衍生物在Chiralpak AD手性固定相上的对映体分离及热力学性质［J］.理化检验-化学分册, 2008,44(11):1017-1020．
【12】苏立强,高玉玲.高效液相色谱手性流动相添加剂法拆分头孢羟氨苄对映体［J］.理化检验-化学分册, 2010,46(3):273-275．
【13】HOU Shi-chong, ZHOU Zhi-qiang, QIAO Zhen. Enantiomer separation of tebuconazole and its potential impurities by high performance liquid chromatography using cellulose derivative-based chiral stationary phase［J］. Chromatographia, 2003,57:177-180．
【14】刘长令.世界农药大全:杀菌剂卷［M］.北京:化学工业出版社, 2008:211-212．
【2】ELLINGTON J J, ENANNS J. High-performance liquid chromatographic separation of the enantiomers of organophosphorus pesticides on polysaccharide chiral stationary［J］. Journal of Chromatography A, 2001,928(1):145-154．
【3】LIU Wei-ping, GAN J, SCHLENK D, et al. Enantioselectivity in environmental safety of current chiral insecticide［J］. Proceedings of the National Academy of Sciences, 2005,102:701-706．
【4】LIU Wei-ping, GAN J, QIN Su-jie. Separation and aquatic toxicity of enantiomers of synthetic pyrethroid insecticides［J］. Chirality, 2005,17:127-133．
【5】OCKENFELS H, KHLER F, MEISE W. Teratogenic effect and stereospecificity of a thalidomide metabolite［J］. Pharmazie, 1976,31:492-493．
【6】WU Y S, LEE H K, LI S F. High-performance chiral separation of fourteen triazole fungicides by sulfated β-cyclodextrin-mediated capillary electrophoresis［J］. Journal of Chromatography A, 2001,912:171-179．
【7】利健文.毛细管电泳手性拆分美托洛尔、阿普洛尔和卡替洛尔对映体［J］.理化检验-化学分册, 2010,46(2):175-177．
【8】TORIBIO L, NOZAL M J. Chiral separation of some triazole pesticides by supercritical fluid chromatography［J］. Journal of Chromatography A, 2004,1046:249-253．
【9】LIU Wei-ping, GAN J. Determination of enantiomers of synthetic pyrethroids in water by solid phase microextraction-enantioselective gas chromatography［J］. Journal of Agriculture and Food Chemistry, 2004,52:736-741．
【10】商永严,孙楠.吡氟禾草灵的高效液相色谱法拆分与测定［J］.理化检验-化学分册, 2008,44(4):319-320．
【11】沈报春,袁建勇.二茂铁衍生物在Chiralpak AD手性固定相上的对映体分离及热力学性质［J］.理化检验-化学分册, 2008,44(11):1017-1020．
【12】苏立强,高玉玲.高效液相色谱手性流动相添加剂法拆分头孢羟氨苄对映体［J］.理化检验-化学分册, 2010,46(3):273-275．
【13】HOU Shi-chong, ZHOU Zhi-qiang, QIAO Zhen. Enantiomer separation of tebuconazole and its potential impurities by high performance liquid chromatography using cellulose derivative-based chiral stationary phase［J］. Chromatographia, 2003,57:177-180．
【14】刘长令.世界农药大全:杀菌剂卷［M］.北京:化学工业出版社, 2008:211-212．
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