Study of Interaction Between m-Nitroaniline and Bovine Serum Albumin Using Fluorospectrophotometry
摘 要
应用荧光光谱法研究间硝基苯胺与牛血清白蛋白的相互作用.激发波长为280 nm,发射波长为342 nm,在pH 7.50的Tris-盐酸缓冲溶液中反应150 min后,间硝基苯胺对牛血清白蛋白的猝灭效果最为明显.289 K,304 K和318 K下的结合常数分别为1.667×104,1.428×104,1.250×104L·mol-1.根据分子间的相互作用力与相关热力学参数间的相互关系,结合间硝基苯胺与牛血清白蛋白相互作用的焓变(ΔH)<0和熵变(ΔS)>0,可推断两者的相互作用力主要为静电作用力.结果表明:间硝基苯胺对牛血清白蛋白的荧光猝灭方式为静态猝灭,最后用紫外吸收光谱法对其作用机理进行了确认.
Abstract
Fluorospectrophotometry was applied to the study the interaction between m-nitroaniline and bovine serum albumin. The maximum quenching effect between m-nitroaniline and bovine serum albumin occurred in Tris-HCl buffer solutions of pH 7.50 after reacting for 30 min. The optimum excitation and emission wavelength were 280 nm and 342 nm respectively. The binding constants at 289,304,318 K were found to be 1.667×104,1.428×104,1.250×104L·mol-1 respectively. Base on the force of interaction between the molecules and correlation among the thermodynamic parameters,as well as the values of ΔH (<0) and ΔS (>0) of the interaction between m-nitroaniline and bovine serum albumin,it was deduced that the force of the reaction was mainly due to electrostatic force. It was found that the fluorescence quenching mechanism was static quenching,which was further confirmed by ultraviolet absorption spectrometry.
中图分类号 O657.3
所属栏目 工作简报
基金项目 山东省优秀中青年科学家科研奖励基金(BS2013DX025);青岛市科技计划基础研究项目(13-1-4-226-jch);青岛农业大学高层次人才科研基金项目(631104)
收稿日期 2014/2/10
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备注蒋爱雯(1990-),女,安徽亳州人,硕士研究生,主要从事仪器分析及分子光谱的研究.
引用该论文: JIANG Ai-wen,DUAN Yan-qing,YUAN Wei-guo,WANG Xiu-zhong. Study of Interaction Between m-Nitroaniline and Bovine Serum Albumin Using Fluorospectrophotometry[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(2): 200~204
蒋爱雯,段艳青,袁维国,王修中. 荧光光谱法研究间硝基苯胺与牛血清白蛋白的相互作用[J]. 理化检验-化学分册, 2015, 51(2): 200~204
被引情况:
【1】曾晓丹,朱 琳,朱宝存, "光谱法研究萘酰亚胺类衍生物与牛血清白蛋白的相互作用",理化检验-化学分册 52, 761-765(2016)
【2】刘建民,曾晓丹,朱宝存,朱琳, "光谱法研究诱惑红与牛血清白蛋白的相互作用",理化检验-化学分册 52, 1251-1256(2016)
【3】陈毅挺,李艳霞,黄露,庄婷娟,林棋, "维生素K3的解离常数测定及其与牛血清白蛋白的相互作用",理化检验-化学分册 52, 61-65(2016)
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【6】朱化雨,刘桂明,张援,等.基于铅(Ⅱ)-二甲酚橙配合物反应光度法测定血清蛋白[J].理化检验-化学分册,2008,44(10):989-993.
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【8】孙洋,樊君,胡晓云,等.荧光素钠与牛血清白蛋白相互作用的荧光光谱研究及其分析应用[J].化学学报,2011,69(8):937-944.
【9】王欢,杨小玲.光谱法研究茜素黄GG与牛血清白蛋白的相互作用[J].光谱实验室,2012,29(4):2291-2295.
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【12】WANG Qi-sui,ZHANG Xiao-lei,ZHOU Xia-lan,et. al. Interaction of different thiol-capped CdTe quantum dots with bovine serum albumin[J]. Journal of Luminescence,2012,132(7):1695-1700.
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