高效液相色谱-串联质谱法测定鸡肉产品中金刚烷胺残留量
HPLC-MS/MS Determination of Residual Amount of Amantadine in Chicken Meat Products
摘 要
应用高效液相色谱-串联质谱法测定鸡肉产品中金刚烷胺的残留量.样品经乙腈提取,以乙腈-水(3+7)溶液作为定容溶液.Phenomenex Kinetex C18色谱柱为分离柱,以不同体积比的0.1%(体积分数)甲酸溶液和乙腈为流动相进行梯度洗脱,采用电喷雾正离子源选择反应监测模式检测.采用基质匹配曲线校正.金刚烷胺的质量浓度在0.005~0.1 mg·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为1.0 μg·kg-1.以空白样品为基体进行加标回收试验,所得回收率在82.5%~94.0%之间,测定值的相对标准偏差(n=6)在6.3%~11%之间.
鸡肉产品
金刚烷胺
HPLC-MS/MS
Chicken meat products
Amantadine
Abstract
HPLC-MS/MS was applied to the determination of residual amount of amantadine in chicken meat products. The sample was extracted with acetonitrile and the sample solution was diluted to constant volume with acetonitrile-water (3+7) solution. Phenomenex Kinetex C18 column was used as stationary phase,and the mixture of 0.1% (φ) formic acid solution and acetonitrile mixed in different ratio was used as mobile phase in gradient elution. ESI+ and selective reaction monitor were adopted in MS/MS. Matrix matching curve was used for calibration. Linear relationship between values of peak area and mass concentration of amantadine was obtained in the range of 0.005-0.1 mg·L-1,with detection limit (3S/N) of 1.0 μg·kg-1. On the base of blank sample,test for recovery was made by standard addition method;values of recovery found were in the range of 82.5%-94.0%,with RSD′s (n=6) in the range of 6.3%-11%.
中图分类号 O657.63
所属栏目 工作简报
基金项目
收稿日期 2014/3/8
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备注庾金良(1988-),男,江苏苏州人,硕士研究生,主要从事食品中兽药残留分析.
引用该论文: YU Jin-liang,ZHANG Xiao-yan,BU Hong-zhong,DING Tao,SHEN Chong-yu,WU Bin,CHEN Hui-lan,YIN Yao,CHEN Lei,Lv Chen. HPLC-MS/MS Determination of Residual Amount of Amantadine in Chicken Meat Products[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(2): 224～227
庾金良,张晓燕,卜洪忠,丁涛,沈崇钰,吴斌,陈慧兰,殷耀,陈磊,吕辰. 高效液相色谱-串联质谱法测定鸡肉产品中金刚烷胺残留量[J]. 理化检验－化学分册, 2015, 51(2): 224～227
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参考文献
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【3】MICHAEL H. FDA prohibits use of antiviral drugs in poultry to help keep drugs efective for humans［EB/OL］. ［2006-06-30］. http://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/2006/ucm108620.htm.
【4】YASUHIKO H,YOUICHI F. Simultaneous determination of the binding of amantadine and its analogues to synthetic melanin by liquid chromatography after precolumn derivatization with dansyl chloride[J]. Journal of Chromatographic Science,2005,43:213-217.
【5】楼永军,陈爽,陶巧凤.毛细管柱气相色谱法测定盐酸金刚烷胺有关物质[J].药物分析杂志,2011,31(3):493-495.
【6】SRIRAM N,SADAYAPPAN V R,THOMAS K H. Visible diode laser induced fluorescence detection for capillary electrophoretic analysis of amantadine in human plasma following precolumn derivatization with Cy5.29.OSu[J]. Journal of Pharmaceutical and Biomedical Analysis,1998,18:411-420.
【7】SUN Hai-xin,CAO Li-min,LIN Hong,et al. Analysis of amantadine residues in chicken using HPLC-MS/MS[J]. Science and Technology of Food Industry,2013,32(12):61-89.
【8】陈慧华,韦敏珏,周炜,等.液相色谱-串联质谱法测定动物组织中金刚烷胺和金刚乙胺残留量[J].质谱学报,2013,34(4):226-232.
【9】云环,张朝晖,罗生亮,等.固相萃取/LC-MS/MS法检测动物源性食品中的金刚烷胺[J].现代仪器,2009(6):42-45.
【10】刘正才,杨方,余孔捷,等.液相色谱-电喷雾串联质谱法同时检测鸡组织中5种抗病毒类药物的残留量[J].色谱,2012,30(12):1253-1259.
【11】云环,崔凤云,严华,等.超高效液相色谱-串联质谱法测定鸡肉中的利巴韦林和金刚烷胺[J].色谱,2013,31(8):724-728.
【12】陈刚,邓晓军,孙锦兰,等.高效液相色谱-串联质谱法同时测定动物源性食品中35种兽药残留量[J].理化检验-化学分册,2014,50(7):809-814.
【13】MAYDA I L,JEFFERY S P,BARTON S I,et al. Multiclass determination and confirmation of antibiotic residues in honey using LC-MS/MS[J]. Journal of Agricultural and Food Chemistry,2008,56:1553-1559.
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