流动注射-化学发光法测定药物制剂及生物流体中盐酸多巴胺
Determination of Dopamine Hydrochloride in Pharmaceutical Preparations and Biological Fluids by Flow Injection Chemiluminescence
摘 要
基于盐酸多巴胺对鲁米诺-牛血清白蛋白体系的化学发光具有显著的猝灭作用,建立了测定盐酸多巴胺的流动注射-化学发光法。试验发现鲁米诺-牛血清白蛋白体系化学发光强度的降低值与盐酸多巴胺质量浓度的对数值呈线性关系,线性范围为0.10~700 μg·L-1,检出限(3σ)为0.03 μg·L-1。方法应用于盐酸多巴胺注射液的测定,加标回收率在97.9%~103%之间,相对标准偏差(n=7)在1.0%~3.2%之间,样品测定值与药典法测定结果一致。本方法还应用于人体唾液、尿液及血清样品中盐酸多巴胺的测定。
鲁米诺
牛血清白蛋白
化学发光
流动注射
Dopamine hydrochloride
Luminol
Bovine serum albumin
Chemiluminescence
Flow injection
Abstract
A flow injection-chemiluminescence (FI-CL) method for the determination of dopamine hydrochloride was developed, based on its marked quenching effect on the chemiluminescence of luminol-bull serum albumin system. Linear relationship was found between the decrement of CL intensity and the logarithm of mass concentration of dopamine hydrochloride ranging from 0.10 to 700 μg·L-1 with the detection limit (3σ) of 0.03 μg·L-1. The proposed method was applied for the determination of dopamine hydrochloride in injections, with values of recovery and RSD (n=7) in the range of 97.9%-103%, 1.0%-3.2% respectively. The determination values of this method were consistent with those of pharmacopeia method. This method was also applied to determine dopamine hydrochloride in human saliva, urine and serum samples.
中图分类号 O657.39
所属栏目 工作简报
基金项目 国家自然科学基金资助项目(20975081);西安医学院博士科研启动项目(2012DOC12);西安医学院大学生科研计划项目(12DXS04);陕西省教育厅科研计划项目(14JK1620)
收稿日期 2014/3/18
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备注张韫(1987-),女,陕西西安人,讲师,硕士,主要从事药物分析与过程分析工作。
引用该论文: ZHANG Yun,GAO Su-ya. Determination of Dopamine Hydrochloride in Pharmaceutical Preparations and Biological Fluids by Flow Injection Chemiluminescence[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(4): 470～473
张韫,高苏亚. 流动注射-化学发光法测定药物制剂及生物流体中盐酸多巴胺[J]. 理化检验－化学分册, 2015, 51(4): 470～473
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参考文献
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【4】LIU Yan-ming, WANG Cheng-quan, MU Hai-bei, et al. Determination of catecholamines by CE with direct chemiluminescence detection［J］. Electrophoresis, 2007,28(12):1937-1941.
【5】GUO Li, ZHANG Yan, LI Quan-min. Spectrophotometric determination of dopamine hydrochloride in pharmaceutical, banana, urine and serum samples by potassium ferricyanide-Fe(Ⅲ)［J］. Analytical Sciences, 2009,25(12):1451-1455.
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【8】DAS S, POWE A M, BAKER G A, et al. Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry［J］. Analytical Chemistry, 2012,84(2):597-625.
【9】WANG Zhu-ming, SONG Zheng-hua, CHEN Dong-hua. Study on the binding behavior of bovine serum albumin with cephalosporin analogues by chemiluminescence method［J］. Talanta, 2010,83(2):312-319.
【10】国家药典委员会.中华人民共和国药典(二部)［M］.北京:中国医药科技出版社, 2010:689.
【2】GUAN Cui-lin, OUYANG Jin, LI Qi-long, et al. Simultaneous determination of catecholamines by ion chromatography with direct conductivity detection［J］. Talanta, 2000,50(6):1197-1203.
【3】周谷珍,孙元喜,黄想成.聚荧光素薄膜修饰电极对多巴胺的电催化作用［J］.理化检验-化学分册, 2006,42(5):331-333.
【4】LIU Yan-ming, WANG Cheng-quan, MU Hai-bei, et al. Determination of catecholamines by CE with direct chemiluminescence detection［J］. Electrophoresis, 2007,28(12):1937-1941.
【5】GUO Li, ZHANG Yan, LI Quan-min. Spectrophotometric determination of dopamine hydrochloride in pharmaceutical, banana, urine and serum samples by potassium ferricyanide-Fe(Ⅲ)［J］. Analytical Sciences, 2009,25(12):1451-1455.
【6】ABDOLMOHAMMAD-ZADEH H, TALLEB Z. Dispersive solid phase micro-extraction of dopamine from human serum using a nano-structured Ni-Al layered double hydroxide, and its direct determination by spectrofluorometry［J］. Microchim Acta, 2012,179(1/2):25-32.
【7】WABAIDUR S M, ALOTHMAN Z A, ALAM S M, et al. Flow injection-chemiluminescence determination of dopamine using potassium permanganate and formaldehyde system［J］. Spectrochim Acta Part A, 2012,96:221-225.
【8】DAS S, POWE A M, BAKER G A, et al. Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry［J］. Analytical Chemistry, 2012,84(2):597-625.
【9】WANG Zhu-ming, SONG Zheng-hua, CHEN Dong-hua. Study on the binding behavior of bovine serum albumin with cephalosporin analogues by chemiluminescence method［J］. Talanta, 2010,83(2):312-319.
【10】国家药典委员会.中华人民共和国药典(二部)［M］.北京:中国医药科技出版社, 2010:689.
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