在碱性环境下,银(Ⅲ)配合物可与鲁米诺产生化学发光,醋酸泼尼松对该发光体系具有显著的增敏作用,据此提出了流动注射银(Ⅲ)配合物-鲁米诺化学发光体系测定醋酸泼尼松含量的方法.优化的试验条件如下:① 鲁米诺溶液中氢氧化钠的浓度为0.6 mol·L-1;② 鲁米诺溶液的浓度为8.0×10-7mol·L-1;③ 银(Ⅲ)配合物溶液中氢氧化钠的浓度为1.7 mol·L-1;④ 银(Ⅲ)配合物溶液的浓度为5.0×10-5mol·L-1.醋酸泼尼松的线性范围为6.0×10-8~8.0×10-5mol·L-1,方法的检出限(3s/k)为2.9×10-9mol·L-1.对1.0×10-6mol·L-1醋酸泼尼松标准溶液连续测定11次,测定值的相对标准偏差为2.9%.加标回收率在100%~105%之间.

In alkaline medium,prednisone acetate could enhance the intensity of chemiluminescence produced by the reaction between Ag(Ⅲ) complexes and luminol.Based on the fact,a method for determination of prednisone acetate by flow injecting Ag(Ⅲ) complexes-luminol chemiluminescence system was proposed.The optimized conditions found were as follows: ① concentration of sodium hydroxide in luminol solution:0.6 mol·L-1;② concentration of luminol solution: 8.0×10-7mol·L-1;③ concentration of sodium hydroxide in Ag(Ⅲ) complexes solution: 1.7 mol·L-1;④ concentration of Ag(Ⅲ) complexes solution: 5.0×10-5mol·L-1.Linearity range of prednisone acetate was found between 6.0×10-8 and 8.0×10-5mol·L-1 with detection limit (3s/k) of 2.9×10-9mol·L-1.1.0×10-6mol·L-1 prednisone acetate standard solution was tested for 11 measurements,value of RSD found was 2.9%.Values of recovery found were in the range of 100%-105%.