顶空-固相微萃取-气相色谱-质谱法测定烟气中挥发性和半挥发性成分
GC-MS Determination of Volatile and Semi-volatile Component in Smoke with Headspace and Solid Phase Microextraction
摘 要
提出了顶空-固相微萃取-气相色谱-质谱法测定烟气中挥发性和半挥发性成分.结合信息量最大的原则,确定了固相微萃取时萃取纤维头为碳分子筛/聚二甲基硅烷萃取纤维头,萃取温度为70 ℃,萃取时间为60 min.在气相色谱分离中用Agilent DB-1色谱柱为固定相,在质谱分析中采用全扫描模式.结果表明:共鉴定出107种成分,含量最大的成分是烟碱(18.07%),其次是新植二烯(11.46%),主要的3类挥发性和半挥发性成分依次为苯系物、酮类以及杂环类.
顶空
固相微萃取
烟气
挥发性
半挥发性
GC-MS
Headspace
Solid phase microextraction
Smoke
Volatile
Semi-volatile
Abstract
A method of GC-MS for the determination of volatile and semi-volatile component in smoke with headspace and solid phase microextraction was proposed.Based on the principle of the greatest amount of information content,carboxen/ polydimeylsiloxane fiber was applied in solid phase microextraction with extraction temperature of 70 ℃ and extraction time of 60 min.Agilent DB-1 chromatographic column was used for GC separation and the full-scanning mode was adopted in MS determination.It was found that 107 components were identified.The highest content was nicotine (18.07%),and the higher content was neophytadiene (11.46%).The main volatile and semi-volatile components were benzene series,ketones,and heterocyclic compounds.
中图分类号 O657.63
所属栏目 工作简报
基金项目 湖南中烟工业有限责任公司项目(2012-JC-002)
收稿日期 2014/5/21
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备注杨琼(1988-),女,湖南长沙人,硕士研究生,研究方向为现代色谱分离分析和烟草香精香料分析.
引用该论文: YANG Qiong,TUO Su-xing,HUANG Yong-bing,ZHAO Yu,YANG Hua-wu,CHEN Bo. GC-MS Determination of Volatile and Semi-volatile Component in Smoke with Headspace and Solid Phase Microextraction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(5): 616～620
杨琼,庹苏行,黄勇兵,赵瑜,杨华武,陈波. 顶空-固相微萃取-气相色谱-质谱法测定烟气中挥发性和半挥发性成分[J]. 理化检验－化学分册, 2015, 51(5): 616～620
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【5】李炎强,冼可法.同时蒸馏萃取法与水蒸气蒸馏法分离分析烟草挥发性、半挥发性中性成分的比较[J].烟草科技,2000(2):18-21.
【6】毛友安,钟科军,卢红兵,等.气相色谱-质谱联用法测定卷烟主流烟气中烟草特有亚硝胺[J].理化检验-化学分册,2008,44(6):529-531.
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【8】郭方遒,钟科军,黄建国,等.烟用香精中风味成分的提取条件的优化与测定[J].分析试验室,2006,25(7):18-22.
【9】GONG Fan,LIANG Yi-zeng,XIE Pei-shan,et al.Information theory applied to chromatographic fingerprint of herbal medicine for quality control[J].Journal of Chromatography A,2003,1002(1):25-40.
【10】俞汝勤.现代分析化学的信息理论基础[J].分析化学,1984,12(7):634-638.
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