以钯(Ⅱ)与吖啶红形成的络合物作为荧光探针荧光光谱法测定L-异亮氨酸
Fluorescence Spectroscopy Determination of L-Isoleucine with Complex of Pd(Ⅱ) and Acridine Red as Fluorescent Probe
摘 要
在pH 10.7的Kolthoff缓冲溶液中,钯(Ⅱ)能与吖啶红形成络合物从而使其荧光发生静态淬灭.当加入L-异亮氨酸后,L-异亮氨酸能与钯(Ⅱ)形成更为稳定的络合物而释放出吖啶红,使体系的荧光强度大幅增加.据此,提出了以钯(Ⅱ)与吖啶红形成的络合物作为荧光探针的荧光光谱法测定L-异亮氨酸的方法.激发和发射光谱通带宽度分别为5,10 nm,反应时间为120 min.L-异亮氨酸的线性范围为0.125~1.375 mg·L-1,方法的检出限(3s/k)为0.27 μg·L-1.L-异亮氨酸与钯(Ⅱ)的络合比为3比1.加标回收率在106%~114%之间,测定值的相对标准偏差(n=6)小于4.0%.
L-异亮氨酸
吖啶红
络合物
Fluorescence spectroscopy
L-isoleucine
Acridine red
Complex
Abstract
In Kolthoff buffer at pH 10.7,Pd(Ⅱ) and acridine red could form a complex,which quenches the fluorescence intensity of acridine red.After adding L-isoleucine,a more stable complex was formed and was free acridine red release,which significantly enhanced the fluorescence intensity of the system.Based on this fact,a fluorospectrophotometric method with fluorescence probe of complex of Pd(Ⅱ) and acridine red was proposed for the determination of L-isoleucine.The excitation slit width was 5 nm and emission slit width was 10 nm.The reaction time was 120 min.The linearity range of L-isoleucine 0.125-1.375 mg·L-1,with detection limit (3s/k) of 0.27 μg·L-1.The complexation ratio of Pd(Ⅱ) to L-isoleucine was 3∶1.The recovery rates measured by standard addition method were in the range of 106%-114%,with RSD (n=6) less than 4.0%.
中图分类号 O657.31
所属栏目 工作简报
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收稿日期 2014/5/22
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备注程定玺(1964-),男,河南固始人,教授,博士,主要研究方向为光分析化学.
引用该论文: CHENG Ding-xi,ZHU Hui-yu,LIU Qi-sheng. Fluorescence Spectroscopy Determination of L-Isoleucine with Complex of Pd(Ⅱ) and Acridine Red as Fluorescent Probe[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(5): 651～654
程定玺,朱会宇,刘起胜. 以钯(Ⅱ)与吖啶红形成的络合物作为荧光探针荧光光谱法测定L-异亮氨酸[J]. 理化检验－化学分册, 2015, 51(5): 651～654
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参考文献
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【5】黄佳华,弓振斌,林继军,等.聚乙烯亚胺-金属络合物的稳定常数及配位数的测定[J].高等学校化学学报,2012,33(12):2633-2637.
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