基于铁氰化钐修饰的玻碳电极研究鸟嘌呤和腺嘌呤的电化学行为及其含量的测定
Study on the Electrochemical Behavior and Simultaneous Determination of Guanine and Adenine with the Samarium Ferricyanide Modified GCE
摘 要
利用电化学沉积法将铁氰化钐沉积在玻碳电极表面,形成铁氰化钐修饰电极,并利用循环伏安法研究了鸟嘌呤和腺嘌呤在该修饰电极上的电化学行为.相比裸玻碳电极,鸟嘌呤和腺嘌呤在修饰电极上的氧化峰电流增大,氧化峰电位降低,说明该修饰电极对鸟嘌呤和腺嘌呤的氧化具有良好的电催化能力.在优化条件下,两者的氧化峰电流与其浓度在5.0~70 μmol·L-1范围内呈现线性关系,检出限(3S/N)均为1.0 μmol·L-1.
铁氰化钐修饰电极
鸟嘌呤
腺嘌呤
Cyclic voltammetry
SmFe(CN)6 modified GCE
Guanine
AdenineDNA
Abstract
Samarium ferricyanide was deposited on the surface of GCE by electrochemical cyclic voltammetry (CV) to prepare the SmFe(CN)6 modified GCE.The electrocatalytic oxidation of guanine and adenine at the modified electrode was studied by CV.As compared with bare GCE,values of oxidation peak current of both guanine and adenine at the modified electrode were found to be increased while the oxidation peak potential to be decreased,showing that the modified electrode had good electrocatalytic activity toward the oxidation of guanine and adenine.Under optimum conditions,linear relationship was found between values of oxidation peak current and concentration of guanine and adenine in the same range of 5.0-70 μmol·L-1,with same detection limit (3S/N) of 1.0 μmol·L-1.
中图分类号 O657.14 DOI 10.11973/lhjy-hx201507003
所属栏目 试验与研究
基金项目 国家自然科学基金项目(21375016,21475022);广东省自然科学基金项目(S2013010014324)
收稿日期 2014/7/28
修改稿日期
网络出版日期
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备注张敏(1978-),女,甘肃天水人,副教授,主要从事电化学传感器方面的研究工作.
引用该论文: ZHANG Min,MENG Yu-yang,LIU Peng,CHENG Fa-liang. Study on the Electrochemical Behavior and Simultaneous Determination of Guanine and Adenine with the Samarium Ferricyanide Modified GCE[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(7): 902～906
张敏,孟煜阳,柳鹏,程发良. 基于铁氰化钐修饰的玻碳电极研究鸟嘌呤和腺嘌呤的电化学行为及其含量的测定[J]. 理化检验－化学分册, 2015, 51(7): 902～906
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【3】杨孝容,万东海,曹秋娥.维多利亚蓝B标记分光光度法测定小牛胸腺DNA,黄连素标记的脱氧核糖核酸荧光毛细生物传感器的研制及应用[J].理化检验-化学分册,2005,41(5):318-320．
【4】陈丹丹,张涛,任丽萍,等.荧光分光光度法测定痕量核酸-基于核酸与表棓儿茶素棓酸酯-铜(Ⅱ)配合物之间的荧光增强反应[J].理化检验-化学分册,2008,44(12):1206-1208．
【5】杨更亮,李海鹰,刘海燕,等.毛细管区带电泳法测定冬虫夏草中的腺苷、腺嘌呤和尿嘧啶毛细管区带电泳法测定冬虫夏草中的腺苷、腺嘌呤和尿嘧啶[J].分析化学,2002,30(9):1081-1084．
【6】El-SAID W A,CHOI J W.Electrochemical biosensor consisted of conducting polymer layer on gold nanodots patterned indium tin oxide electrode for rapid and simultaneous determination of purine bases[J].Electrochimica Acta,2014,123:51-57．
【7】WANG Hui-sheng,JU Huang-xian,CHEN Hong-yuan.Simultaneous determination of guanine and adenine in DNA using an electrochemically pretreated glassy carbon electrode[J].Anal Chim Acta,2002,461(2):243-250．
【8】张敏,程发良,蔡志泉.纳米金修饰玻碳电极对鸟嘌呤的催化氧化[J].分析测试学报,2010,29(7):717-720．
【9】LI Chun-xiang,QIU Xi-yang,LING Yu-ling.Electrocatalytic oxidation and the simultaneous determination of guanine and adenine on (2,6-pyridinedicarboxylic acid)/graphene composite film modified electrode[J].Journal of Electroanalytical Chemistry,2013,704:44-49．
【10】JEEVAGAN A J,JOHN S A.Electrochemical determination of guanosine 5′-monophosphate using the electropolymerized film of non-peripheral amine substituted nickel(Ⅱ) phthalocyanine modified electrode[J].Electrochimica Acta,2013,95:246-250．
【11】钟苑雯,林谋宏,周洁丹,等.铁氰化镨修饰的电化学传感器的研制及其对半胱氨酸的测定[J].分析化学,2010,38(2):229-232．
【12】马扬,王忠德,郝晓刚,等.聚中性红/铁氰化镍/碳纳米管修饰电极的制备及其电催化过氧化氢的特性[J].功能材料,2014,45(1):1129-1134．
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