高效液相色谱法测定进口葡萄酒中的白藜芦醇及其甙
Determination of Resveratrol and Piceid in Imported Wine by HPLC
摘 要
采用高效液相色谱法测定进口葡萄酒中的白藜芦醇及其甙.葡萄酒样品经过C18固相萃取柱净化后,采用Agilent Eclipse XDB-C18色谱柱进行分离,以乙腈溶液为流动相进行梯度洗脱,采用光电二极管阵列检测器在288 nm和306 nm处进行检测.白藜芦醇及其甙的质量浓度在0.2~20 mg·L-1范围内与其峰面积呈线性关系,反式白藜芦醇甙、反式白藜芦醇、顺式白藜芦醇甙和顺式白藜芦醇的测定下限分别为2.6,1.5,4.6,3.0 ng.加标回收率在77.3%~104%之间,测定值的相对标准偏差在1.4%~8.3%之间.
白藜芦醇
白藜芦醇甙
葡萄酒
HPLC
Resveratrol
Piceid
Wine
Abstract
HPLC was used for the determination of resveratrol and piceid in imported wine.The sample was purified by the C18 solid phase extraction column.The extract was separated on an Agilent Eclipse XDB-C18 column with acetonitrile solution as the mobile phase for gradient elution.DAD detection was made at the wavelength of 288,306 nm.The linear relationship was found between the peak area and the mass concentration of resveratrol and piceid in the range of 0.2-20 mg·L-1,and the lower limits of determination of trans-piceid,trans-resveratrol,cis-piceid and cis-resveratrol were 2.6,1.5,4.6,3.0 ng,respectively.The recovery rates measured by standard addition method were in the range of 77.3%-104%,with RSD in the range of 1.4%-8.3%.
中图分类号 O657.7 DOI 10.11973/lhjy-hx201507011
所属栏目 工作简报
基金项目 浙江省重点科技创新团队项目(2010R50028);质检总局科技计划项目(2013IK188)
收稿日期 2014/7/11
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备注侯建波(1983-),男,(满族)内蒙古人,工程师,博士,研究方向食品中药物残留分析.
引用该论文: HOU Jian-bo,XIE Wen,SHI Ying-zhu,SHEN Wei-feng,He Jian-min. Determination of Resveratrol and Piceid in Imported Wine by HPLC[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(7): 938～942
侯建波,谢文,史颖珠,沈炜锋,何建敏. 高效液相色谱法测定进口葡萄酒中的白藜芦醇及其甙[J]. 理化检验－化学分册, 2015, 51(7): 938～942
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参考文献
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【3】宋于洋,汪宗玉,杨新民,等.葡萄酒中的白藜芦醇[J].酿酒,2003,30(6):44-46．
【4】黄卫文,黄寿恩,黎继烈,等.白藜芦醇的提取、检测技术及其生物活性研究进展[J].食品与机械,2010,26(6):148-152．
【5】LUAN Tian-gang,LI Gong-ke,ZHANG Zhan-xia.Gas-phase postderivatization following solid-phase microextraction for rapid determination of trans-resveratrol in wine by gas chromatography-mass spectrometry[J].Analytica Chimica Acta,2000,424(1):19-25．
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【7】FLAMINI R,VEDOVA A D.Fast determination of the total free resveratrol content in wine by direct-exposure-probe,positive-ion chemical ionization and collision-induced-dissociation mass spectrometry[J].Rapid Communications in Mass Spectrometry,2004,18(17):1925-1931．
【8】PINEIRO Z,PALMA M,BARROSO C G.Determination of trans-resveratrol in grapes by pressurised liquid extraction and fast high-performance liquid chromatography[J].Journal of Chromatography A,2006,1110(1/2):61-65．
【9】LOPEZ R,DUGO P,MONDELLO L.Determination of trans-resveratrol in wine by micro-HPLC with fluorescence detection[J].Journal of Separation Science,2007,30(5):669-672．
【10】GURBUZ O,GOCMEN D,DAGDELEN F,et al.Determination of flavan-3-ols and trans-resveratrol in grapes and wine using HPLC with fluorescence detection[J].Food Chemistry,2007,100(2):518-525．
【11】GONCALVES J,CAMARA J.New method for determination of (E)-resveratrol in wine based on microextraction using packed sorbent and ultra-performance liquid chromatography[J].Journal of Separation Science,2011,34(18):2376-2384．
【12】刘芳华,彭友元,叶建农.毛细管电泳-电化学检测法测定葡萄和葡萄酒中的白藜芦醇[J].分析测试学报,2005,24(3):125-127．
【13】SPANILA M,PAZOUREK J,FARKOVA M,et al.Optimization of solid-phase extraction using artificial neural networks in combination with experimental design for determination of resveratrol by capillary zone electrophoresis in wines[J].Journal of Chromatography A,2005,1084(1/2):180-185．
【14】陈敏,舒友琴,何计国,等.薄层荧光扫描法测定葡萄酒中的白藜芦醇及其糖甙异构体[J].分析化学,2005,33(5):635-638．
【15】GAMOH K,NAKASHIMA K.Liquid chromatography/mass spectrometric determination of trans-resveratrol in wine using a tandem solid-phase extraction method[J].Rapid Communications in Mass Spectrometry,1999,13(12):1112-1115．
【16】刘达雄,栾玉明,区伟全,等.国产葡萄酒中白藜芦醇含量分析[J].中国卫生检验杂志,2008,18(12):2813-2814．
【17】李栋.HPLC法测定酒中顺、反式白藜芦醇含量的研究[J].湖北师范学院学报,2008,28(1):92-94．
【18】HERNANDEZ J L,LOSADA P P,SILVA A T S,et al.Study of the changes of trans-resveratrol caused by ultraviolet light and determination of trans- and cis-resveratrol in Spanish white wines[J].European Food Research and Technology,2007,225(5/6):789-796．
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