采用气相色谱-质谱法测定大鼠血浆中的尼古丁.血浆样品采用蛋白沉淀法净化,采用DB-Wax毛细管色谱柱分离,质谱中选择电子轰击离子源和选择离子监测模式,以茴香脑为内标进行定量.并采用PKSlover 2.0软件计算药代动力学参数.尼古丁的线性范围为0.10~2.0 mg·L-1,检出限为4.59 μg·L-1.对空白样品进行加标回收试验,回收率在85.0%~90.1%之间,相对标准偏差(n=6)小于3.0%.应用此方法考察了灌胃给药的大鼠血浆样品中尼古丁代谢动力学特点,主要动力学参数AUC0-t,AUC0-∞,Tmax和ρmax分别为8.570 mg·L-1·h-1,10.49 mg·L-1·h-1,1.0 h,998.9 μg·L-1,结果呈现药物代谢动力学过程.

GC-MS was used for the determination of nicotine in rat plasma. The plasma sample was purified by a protein precipitation method and then separated on a DB-Wax capillary column. EI and SIM were adopted in MS. Anethole was used as the internal standard for quantification. Pharmacokinetic parameters were calculated by using software PKSlover 2.0. The linearity range of nicotine was 0.10-2.0 mg·L-1 with detection limit of 4.59 μg·L-1．The recovery rates measured by standard addition method were in the range of 85.0%-90.1% with RSD less than 3.0%. The method was applied for pharmacokinetic study of nicotine in the rat plasma samples. AUC0-t,AUC0-∞,Tmax and ρmax were 8. 570 mg·L-1·h-1,10.49 mg·L-1·h-1,1.0 h,and 998.9 μg·L-1,respectively,displaying a pharmacokinetic process.