石墨烯修饰玻碳电极用于循环伏安法测定盐酸表阿霉素
Determination of Epirubicin Hydrochloride by Cyclic Voltamming Using Graphene Modified Glassy Carbon Electrode
摘 要
将石墨烯修饰在玻碳电极表面用于循环伏安法测定盐酸表阿霉素.在pH 4.0的B-R缓冲溶液中,在修饰电极上,盐酸表阿霉素在-0.382 V处可见明显的氧化峰,且氧化峰电流比在裸玻碳电极上提高两倍以上.盐酸表阿霉素的氧化峰电流与其浓度在1.0×10-7~1.0×10-6mol·L-1范围内呈线性关系,检出限(3S/N)为2.0×10-9mol·L-1.方法用于盐酸表阿霉素注射液的测定,加标回收率在95.9%~97.7%之间,测定值的相对标准偏差(n=6)小于2%.
石墨烯修饰电极
盐酸表阿霉素
Cyclic voltammetry
Gaphene modified electrode
Epirubicin hydrochloride
Abstract
Graphene modified glassy carbon electrode was used for determination of epirubicin hydrochloride by cyclic voltammetry. In the B-R solution with pH 4.0,epirubicin hydrochloride showed an obvious oxidation peak at -0.382 V on the graphene modified electrode,and the current of oxidation peak on the modified electrode was two times more than that on the bare glassy carbon electrode. The linear relationship was found between the oxidation peak current and the concentration of epirubicin hydrochloride in the range of 1.0×10-7-1.0×10-6mol·L-1 with the detection limit (3S/N) was 2.0×10-9mol·L-1 .The method was used for analysis of epirubicin hydrochloride injection,giving recovery rates measured by standard addition method in the range of 95.9%-97.7% and RSD (n=6) was less than 2%.
中图分类号 O657.14 DOI 10.11973/lhjy-hx201507034
所属栏目 专题报道(药物分析)
基金项目 山西省青年科技基金项目(20021008)
收稿日期 2014/7/26
修改稿日期
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备注张勇(1963-),男,山西芮城人,教授,主要研究方向为药物分析.
引用该论文: ZHANG Yong,DU Hui-lin,CHENG Hong-fen,WANG Ying-te. Determination of Epirubicin Hydrochloride by Cyclic Voltamming Using Graphene Modified Glassy Carbon Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(7): 1028～1031
张勇,杜慧琳,程红芬,王英特. 石墨烯修饰玻碳电极用于循环伏安法测定盐酸表阿霉素[J]. 理化检验－化学分册, 2015, 51(7): 1028～1031
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参考文献
【1】CHEN Zhe,DRAMOU P,HE Hua,et al. Adsorption behavior of epirubicin hydrochloride on carboxylated carbon nanotubes[J]. International Journal of Pharmaceutics,2011,405(1/2):153-161．
【2】杨翠.金纳米粒子修饰玻碳电极及其检测表阿霉素的研究[D].沈阳:沈阳师范大学,2012．
【3】PUMERA M,AMBROSI A,BONANNI A,et al. Graphene for electrochemical sensing and biosensing[J]. TrAC Trends in Analytical Chemistry,2010,29(9):954-965．
【4】赖丽燕,刘峥,袁帅.石墨烯在生物传感器中的应用[J].理化检验-化学分册,2013,49(3):370-375．
【5】REINHOUD N J,TJADEN U R,IRTH H,et al. Bioanalysis of some anthracyclines in human plasma by capillary electrophoresis with laser-induced fluorescence detection[J]. Journal of Chromatography,1992,574(2):327-334．
【6】龚晓丽,秦永平,梁茂,等.HPLC荧光和质谱两种检测器测定大鼠血浆及组织中的表阿霉素[J].华西药学杂志,2008,23(1):68-70．
【7】FERRERO J M,ETIENNE M C,FORMENTO J L,et al. Application of an original RT-PCR-ELISA multiplex assay for MDR1 and MRP,along with p53 determination in node-positive breast cancer patients[J]. British Journal of Cancer,2000,82(1):171-177．
【8】MARCZAK A. Fluorescence anisotropy of membrane fluidity probes in human erythrocytes incubated with anthracyclines and glutaraldehyde[J]. Bioelectrochemistry,2009,74(2):236-239．
【9】吴燕,张福成,吴诚,等.盐酸表阿霉素长循环热敏脂质体大鼠药代动力学考察[J].药学学报,2010,45(3):365-370．
【10】BOZAL-PALABIYIK B,KURBANOGLU S,GUMUSTAS M,et al. Electrochemical approach for the sensitive of anticancer drug epirubicin in pharmaceuticals in the presence of anionic surfactant[M]. Revue Roumaine de Chimie,2013,58(7/8):647-658．
【11】叶雪梅,林聪丽,汤从容.循环伏安法测定输液中表柔比星的含量[J].中国药师,2011,14(5):642-643．
【12】程红芬.盐酸表阿霉素的光谱与电化学研究及其分析应用[D].太原:山西大学,2011．
【13】张京京.碳纳米管修饰电极在生物及药物分析中的应用[D].聊城:聊城大学,2007．
【14】KANO K,KONSE T,NISHIMURA N,et al. Electrochemical properties of adriamycin adsorbed on a mercury electrode surface[J]. Bulletin of the Chemical Society of Japan,1984,57(9):2383-2390．
【15】于金海.石墨烯的非共价功能化修饰及载药系统研究[D].青岛:青岛科技大学,2012．
【16】BERG H,HORN G,LUTHARDT U,et al. Interaction of anthracycline antibiotics with biopolymers: Part V. Polarographic behavior and complexes with DNA[J]. Bioelectrochemistry and Bioenergetics,1981,8(5):537-553．
【2】杨翠.金纳米粒子修饰玻碳电极及其检测表阿霉素的研究[D].沈阳:沈阳师范大学,2012．
【3】PUMERA M,AMBROSI A,BONANNI A,et al. Graphene for electrochemical sensing and biosensing[J]. TrAC Trends in Analytical Chemistry,2010,29(9):954-965．
【4】赖丽燕,刘峥,袁帅.石墨烯在生物传感器中的应用[J].理化检验-化学分册,2013,49(3):370-375．
【5】REINHOUD N J,TJADEN U R,IRTH H,et al. Bioanalysis of some anthracyclines in human plasma by capillary electrophoresis with laser-induced fluorescence detection[J]. Journal of Chromatography,1992,574(2):327-334．
【6】龚晓丽,秦永平,梁茂,等.HPLC荧光和质谱两种检测器测定大鼠血浆及组织中的表阿霉素[J].华西药学杂志,2008,23(1):68-70．
【7】FERRERO J M,ETIENNE M C,FORMENTO J L,et al. Application of an original RT-PCR-ELISA multiplex assay for MDR1 and MRP,along with p53 determination in node-positive breast cancer patients[J]. British Journal of Cancer,2000,82(1):171-177．
【8】MARCZAK A. Fluorescence anisotropy of membrane fluidity probes in human erythrocytes incubated with anthracyclines and glutaraldehyde[J]. Bioelectrochemistry,2009,74(2):236-239．
【9】吴燕,张福成,吴诚,等.盐酸表阿霉素长循环热敏脂质体大鼠药代动力学考察[J].药学学报,2010,45(3):365-370．
【10】BOZAL-PALABIYIK B,KURBANOGLU S,GUMUSTAS M,et al. Electrochemical approach for the sensitive of anticancer drug epirubicin in pharmaceuticals in the presence of anionic surfactant[M]. Revue Roumaine de Chimie,2013,58(7/8):647-658．
【11】叶雪梅,林聪丽,汤从容.循环伏安法测定输液中表柔比星的含量[J].中国药师,2011,14(5):642-643．
【12】程红芬.盐酸表阿霉素的光谱与电化学研究及其分析应用[D].太原:山西大学,2011．
【13】张京京.碳纳米管修饰电极在生物及药物分析中的应用[D].聊城:聊城大学,2007．
【14】KANO K,KONSE T,NISHIMURA N,et al. Electrochemical properties of adriamycin adsorbed on a mercury electrode surface[J]. Bulletin of the Chemical Society of Japan,1984,57(9):2383-2390．
【15】于金海.石墨烯的非共价功能化修饰及载药系统研究[D].青岛:青岛科技大学,2012．
【16】BERG H,HORN G,LUTHARDT U,et al. Interaction of anthracycline antibiotics with biopolymers: Part V. Polarographic behavior and complexes with DNA[J]. Bioelectrochemistry and Bioenergetics,1981,8(5):537-553．
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