采用毛细管区带电泳法测定替吉奥胶囊中的替加氟、吉美嘧啶和奥替拉西钾的含量.采用弹性石英毛细管柱为分离柱,以pH 11.8的20 mmol·L-1磷酸氢二钠溶液为运行缓冲溶液,分离温度为20 ℃,分离电压为30 kV,测定波长为240 nm.替加氟和奥替拉西钾在50~500 mg·L-1,吉美嘧啶在14.5~145 mg·L-1范围内呈线性,检出限分别为1.0,2.3,0.6 μg·L-1.3种化合物的加标回收率在98.2%~100%之间,测定值的相对标准偏差(n=9)在0.4%~0.9%之间.该方法用于替吉奥胶囊的分析,测定值与标示值相符.

A method of capillary zone electrophoresis was developed for the determination of ftoafur,gimeracil and potassium oxonate in Tijiao capsules. An elastic quartz capillary tube was used for separation at 20 ℃ and the separate voltage of 30 kV with a running buffer of Na2HPO4 (2 mmol·L-1,pH=11.8). The detection wavelength was 220 nm. The linearity ranges of ftoafur and passium oxonateot were 50-500 mg·L-1 and the linearity range of gimeracil was 14.5-145 mg·L-1. The detection limits were 1.0,2.3,0.6 μg·L-1,respectively. The recovery rates measured by standard addition method were in the range of 98.2%-100%,and RSD (n=9) were in the range of 0.4%-0.9%.The method was used for analyzing Tijiao capsules,giving results in agreement with the indicated value.