聚L-精氨酸/纳米金修饰电极测定多巴胺
Determination of Dopamine with Poly(L-argenine)/Gold Nanoparticles Modified Electrode
摘 要
利用多电位脉冲沉积法制备纳米金修饰电极(AuNPs/GCE),再将L-精氨酸电聚合在AuNPs/GCE表面,制备出一种新型的聚L-精氨酸/AuNPs/GCE.采用原子力显微镜对上述电极进行了表征,并研究了多巴胺在其上的电化学行为.结果表明:在pH 5.7的磷酸盐缓冲溶液中,聚L-精氨酸/AuNPs/GCE对多巴胺的氧化有良好的电催化作用,多巴胺的氧化还原反应是受吸附控制的准可逆过程.多巴胺的浓度在8.0×10-7~1.0×10-4mol·L-1范围内与其氧化峰电流呈线性关系,检出限(3S/N)为1.0×10-7mol·L-1.加标回收率在96.5%~104%之间.对3.0×10-5mol·L-1多巴胺溶液连续测定7次,峰电流的相对标准偏差为2.6%.
聚L-精氨酸
多巴胺
修饰电极
Gold nanoparticles
Poly(L-argenine)
Dopamine
Modified electrode
Abstract
A novel poly(L-argenine)/gold nanoparticles modified electrode (AuNPs/GCE) was fabricated by electropolymerization of poly(L-argenine)/AuNPs/GCE which was prepared by AuNPs/GCE.The modified electrode was characterized by atomic force microscopy.The electrochemical behavior of dopamine on the poly(L-argenine)/AuNPs/GCE was studied.It was found that the poly(L-argenine)/AuNPs/GCE exhibited strong electrocatalytic activity to the oxidation of dopamine in phosphate buffer solution of pH 5.7,and the redox reaction of dopamine was a quasi-reversible process controlled mainly by adsorption.Linear relationship between values of oxidation peak current and concentration of dopamine was kept in the range of 8.0×10-7-1.0×10-4mol·L-1,with detection limits (3S/N) of 1.0×10-7mol·L-1.The proposed method was applied to the analysis of samples of dopamine injection,giving values of recovery in the range of 96.5%-104%.Precision of the method was tested at the concentration level of 3.0×10-5mol·L-1 dopamine solution for 7 determination,with value of RSD of peak current of 2.6%.
中图分类号 O657.14 DOI 10.11973/lhjy-hx201508002
所属栏目 试验与研究
基金项目 国家自然科学基金项目(21305085;21175091);河南省高校科技创新团队支持计划与河南省科技创新型科技人才队伍建设工程资助(2012IRTSTHN018)
收稿日期 2014/8/5
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联系人作者朱旭(ocina@yeah.net)
备注朱旭(1981-),男,辽宁鞍山人,副教授,博士,研究方向为生化分析及生物传感器.
引用该论文: ZHU Xu,WU Shu-fang,LI Chun-lan,XU Mao-tian,YE Bao-xian. Determination of Dopamine with Poly(L-argenine)/Gold Nanoparticles Modified Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(8): 1057～1062
朱旭,吴淑芳,李春兰,徐茂田,冶保献. 聚L-精氨酸/纳米金修饰电极测定多巴胺[J]. 理化检验－化学分册, 2015, 51(8): 1057～1062
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参考文献
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【2】TOBLER P N,FIORILLO C D,SCHULTA W.Adaptive coding of reward value by dopamine[J].Neurons Science,2005,307:1642-1645．
【3】HYMAN S E,MALENKA R C.Addiction and the brain:the neurobiology of compulsion and its persistence[J].Nat Rev Neurosci,2001,2(10):695-703．
【4】LI Na,GUO Ji-zhao,LIU Bo,et al.Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence[J].Anal Chim Acta,2009,645(1/2):48-55．
【5】CARRERA V,SABATER E,VILANOVA E,et al.A simple and rapid HPLC-MS method for the simultaneous determination of epinephrine,norepinephrine,dopamine and 5-hydroxytryptamine:application to the secretion of bovine chromaffin cell cultures[J].J Chromatogr B,2007,847(2):88-94．
【6】WANG Ai-jun,FENG Jiu-ju,DONG Wen-ju,et al.Spermine-graft-dextran non-covalent copolymer as coating material in separation of basic proteins and neurotransmitters by capillary electrophoresis[J].J Chromatogr A,2010,1217(31):5130-5136．
【7】LI Quan-min,LI Juan,YANG Zhan-jun.Study of the sensitization of tetradecyl benzyl dimethyl ammonium chloride for spectrophotometric determination of dopamine hydrochloride using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent[J].Anal Chim Acta,2007,583(1):147-152．
【8】LEE H C,CHEN T H,TSENG W L,et al.Novel core etching technique of gold nanoparticles for colorimetric dopamine detection[J].Analyst,2012,137(22):5352-5357．
【9】YU Da-jun,ZENG Yan-bo,QI Yan-xia,et al.A novel electrochemical sensor for determination of dopamine based on AuNPs@SiO2 core-shell imprinted composite[J].Biosens Bioelectron,2012,38(1):270-277．
【10】ROBINSON D L,HERMANS A,SEIPEL A T,et al.Monitoring rapid chemical communication in the brain[J].Chem Rev,2008,108(7):2554-2584．
【11】MO Jian-wei,OGOREVC B.Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly(1,2-phenylenediamine)-coated carbon fiber[J].Anal Chem,2001,73(6):1196-1202．
【12】DOMENECH A,GARCIA H,DOMENECH-CARBO M T,et al.2,4,6-Triphenylpyrylium ion encapsulated into zeolite Y as a selective electrode for the electrochemical determination of dopamine in the presence of ascorbic acid[J].Anal Chem,2002,74(3):562-569．
【13】马伟,孙登明.聚L-精氨酸修饰电极存在下同时测定多巴胺和肾腺上素[J].分析化学,2007,35(1):66-70．
【14】杜秋香,徐琴,胡效亚.聚合精氨酸修饰玻碳电极在抗坏血酸存在条件下测定多巴胺[J].化学传感器,2008,28(2):39-45．
【15】MARTIN C R,MITCHELL D T.Peer reviewed:nanomaterials in analytical chemistry[J].Anal Chem,1998,70(9):322A-327A．
【16】徐国良,李羚,杨光明.金纳米管阵列修饰玻碳电极用于示差脉冲伏安法测定多巴胺[J].理化检验-化学分册,2012,48(12):1470-1477．
【17】张明明,曹小红,黄平弟.自组装纳米金的多壁碳纳米管微电极的制备及其在测定多巴胺中的应用[J].理化检验-化学分册,2012,48(3):253-256．
【18】蔡称心,鞠熀先,陈洪渊.聚硫堇修饰微带金电极的性质及对NADH的催化氧化[J].高等学校化学学报,1995,16(3):368-372．
【19】方惠群,侯士峰,陈洪渊.乙撑二氧噻吩在中性水溶液中的电化学聚合及行为研究[J].化学学报,1995,53(7):710-715．
【20】樊雪梅,王书民,赵夏,等.聚对氨基苯磺酸修饰电极差分脉冲伏安法测定多巴胺[J].理化检验-化学分册,2010,46(8):917-922．
【21】LAVIRON E.Adsorption,autoinhibition and autocatalysis in polarography and in linear potential sweep voltammetry[J].J Electroanal Chem Interfacial Electrochem,1974,52(3):355-393．
【22】WU Kang-bing,FEI Jun-jie,HU Sheng-shui.Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes[J].Anal Biochem,2003,318(1):100-106．
【23】孙登明,张振新,马伟,等.聚L-酪氨酸修饰电极的制备及对多巴胺的测定[J].分析试验室,2005,24(7):28-31．
【24】AOKI K,AKIMOTO K,TOKUDA K,et al.Linear sweep voltammetry at very small stationary disk electrodes[J].J Electroanal Chem Interfacial Electrochem,1984,171(1/2):219-230．
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