对皂角刺多糖的提取及抗氧化活性进行了研究.采用超声波辅助技术提取皂角刺中的多糖,用苯酚-硫酸法,以葡萄糖为标准对照物在波长484 nm处测量吸光度,计算皂角刺中多糖含量.单因素试验和L9(34)正交试验得到的优化试验条件为:提取温度50 ℃、pH为7、提取时间40 min、液料比为30比1.皂角刺中多糖的提取率为1.26%.抗氧化试验结果表明:当皂角刺多糖质量浓度为2.5 g·L-1时,·OH、DPPH·及·O2-的清除率分别为88.9%,89.3%,42.3%.

Extraction and antioxidation of polysaccharides in Chinese honeylocust spine was studied.The polysaccharide content of Chinese honeylocust spine was extracted with ultrasonic assisted technology and determined by phenol-sulfuric acid method using glucose as standard reference at the wavelength of 484 nm.The optimized conditions found by a series single-factor test and L9(34) orthogonal experiment were as follows:extraction temperature was 50 ℃;pH was 7;extraction time was 40 min and ratio of liquid to solid was 30∶1.Extraction yield of polysaccharide in Chinese honeylocust spine found was 1.26%.The antioxidative experiments indicated that removal rates of ·OH,DPPH· and ·O2- found were 88.9%,89.3% and 42.3%,respectively at mass concentration of polysaccharide in Chinese honeylocust spine of 2.5 g·L-1.