采用高效液相色谱法测定指甲油中甲醛、乙醛和丙酮的含量.样品用乙腈和水提取,2,4-二硝基苯肼为衍生剂,采用Kromasil-C18色谱柱为分离柱,以乙腈-水(65+35)溶液为流动相,在检测波长364 nm处进行测定.甲醛、乙醛和丙酮的质量浓度均在0.01~20.0 mg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.001 5~0.003 5 mg·L-1之间.加标回收率在88.6%~110%之间,相对标准偏差(n=6)在1.2%~9.7%之间.

HPLC was applied to the determination of formaldehyde,acetaldehyde and acetone in nail polish.The sample was extracted with acetonitrile and water with 2,4-dinitrophenylhydrazine as derivative agent.Kromasil-C18 chromatographic column was used as stationary phase and a mixture of acetonitrile and water (65+35) was used as mobile phase.UV-detection at 364 nm was adopted in the determination.Linear relationships between values of peak area and mass concentration of formaldehyde,acetaldehyde and acetone were kept in the same range of 0.01-20.0 mg·L-1,with detection limits (3S/N) in the range of 0.001 5-0.003 5 mg·L-1.Values of recovery found by standard addition method were in the range of 88.6%-110%,and values of RSD′s (n=6) in the range of 1.2%-9.7%.