采用气相色谱-质谱法测定主流烟气中的烟碱、降烟碱、麦斯明、假木贼碱和新烟草碱。捕集有总粒相物的剑桥滤片加入内标后,用50 g·L-1氢氧化钠溶液将植物碱游离出来,经乙酸乙酯萃取后,在DB-35MS色谱柱上分离,采用选择离子扫描模式。5种植物碱的浓度与峰面积在一定范围内呈线性关系。烟碱的检出限(3S/N)为12 μg·支-1,其他4种植物碱的检出限(3S/N)为0.30~2.5 μg·支-1。5种植物碱的加标回收率在86.5%~108%之间,测定值的相对标准偏差(n=6)在1.7%~4.5%之间。

GC/MS was applied to the determination of 5 alkaloids, including nicotine, nornicotine, myosmine, anabasine and anatabine, in mainstreams of cigarette smoke. The total particulates in the smoke were collected on cambridge filter pad, and after addition of internal standard, the filter pad was treated with 50 g·L-1 NaOH solution to liberate the alkaloids. The solution was then extracted with ethyl acetate and a separation was made on DB-35MS column. SIM was adopted in MS. Linear relationships were found between values of peak areas and concentrations of the 5 alkaloids in definite ranges. Values of detection limits (3S/N) found for nicotine was 12 μg·cig-1, and for the other 4 alkaloids were in the range of 0.30-2.5 μg·cig-1. Values of recovery found by standard addition method were in the range of 86.5%-108%, and RSD′s (n=6) in the range of 1.7%-4.5%.