提出了一种测定氮丙啶的高效液相色谱分析方法。将自制氮丙啶单体与福林试剂(1,2-萘醌-4-磺酸钠盐)反应得到衍生物,通过NMR和IR表征验证衍生物结构。衍生物在Agilent ODB C18色谱柱(250 mm×4.6 mm,5 μm)上分离,以乙腈-0.04 mol·L-1磷酸氢二钠混合溶液为流动相进行梯度洗脱,检测波长为258 nm。氮丙啶的线性范围为1.00~20.0 mg·L-1,检出限(3S/N)为0.05 mg·L-1,测定值的相对标准偏差(n=6)小于2%。

A HPLC was applied to the determination of aziridine. Self-made aziridine monomer was derivatized with Folin′s reagent (1,2-naphthoquione-4-sulphonic acid) and the structure of the derivate was characterized by NMR and IR. For determination of aziridine, the derivate was seperated on Agilent ODB-C18 (250 mm×4.6 mm, 5 μm) column, using a mixtrure of acetonitrile and 0.04 mol·L-1 sodium phosphate dibasic dodecahydrate solution as mobile phase in gradient elution. The wavelength used in UV-detection was 258 nm. The linearity range for aziridine was in the range of 1.00-20.0 mg·L-1, the detection limit (3S/N) was 0.05 mg·L-1, and RSD′s (n=6) found were less than 2%.