气相色谱-质谱法测定土壤中的磷脂脂肪酸
GC-MS Determination of Phospholipid Fatty Acids in Soil
摘 要
采用气相色谱-质谱法测定土壤中的磷脂脂肪酸。土壤样品加入氢氧化钾的甲醇溶液中,其中的磷脂脂肪酸经甲酯化后,用正己烷萃取.萃取液采用Agilent DB-5MS色谱柱分离,质谱分析中选用全扫描和选择离子监测模式。26种脂肪酸甲酯的质量浓度在一定范围内与峰面积呈线性关系,检出限(3S/N)在0.001 2~0.295 9 mg·L-1之间。加标回收率在108%~115%之间,测定值的相对标准偏差(n=7)在3.2%~6.4%之间。
磷脂脂肪酸
土壤
GC-MS
Phospholipid fatty acid
Soil
Abstract
GC-MS was applied to the determination of phospholipid fatty acids in soil. The sample was added into KOH solution with methanol as solvent. After methyl esterification, fatty acid methyl esters were extracted with hexane. The extract was separated on an Agilent DB-5MS column. Full-scaning mode and SIM were adopted in MS. Linear relationships were found between the peak area and the mass concentration of the 26 fatty acids methyl esters in definite ranges, with detection limits (3S/N) in the range of 0.001 2-0.295 9 mg·L-1. Recovery rates measured by standard addition method were in the range of 108%-115%, and RSDs (n=7) were in the range of 3.2%-6.4%.
中图分类号 O657.63 DOI 10.11973/lhjy-hx201510001
所属栏目 试验与研究
基金项目 国家“十二五”水专项(2012ZX07101007-005);国家自然科学基金(31123001;51208498;51308530)。
收稿日期 2014/10/9
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备注张丽萍(1979-),女,山西太原人,实验师,硕士,主要从事环境污染修复方面的研究。
引用该论文: ZHANG Li-ping,WANG Chuan,ZHOU Qiao-hong,WANG Ya-fen,WU Zhen-bin. GC-MS Determination of Phospholipid Fatty Acids in Soil[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(10): 1353～1357
张丽萍,王川,周巧红,王亚芬,吴振斌. 气相色谱-质谱法测定土壤中的磷脂脂肪酸[J]. 理化检验－化学分册, 2015, 51(10): 1353～1357
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参考文献
【1】王曙光,侯彦林.磷脂脂肪酸方法在土壤微生物分析中的应用［J］.微生物学通报, 2004,31(1):114-117.
【2】KAUR A, CHAUDHARY A, KAUR A, et al. Phospholipid fatty acid-A bioindicator of environment monitoring and assessment in soil ecosystem［J］. Current Science, 2005,89(7):1103-1112.
【3】BUYER J S, SASSER M. High throughput phospholipid fatty acid analysis of soils［J］. Applied Soil Ecology, 2012,61(S1):127-130.
【4】齐鸿雁,薛凯,张洪勋.磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用［J］.生态学报, 2003,23(8):1576-1582.
【5】张瑞娟,李华,林勤保,等.土壤微生物群落表征中磷脂脂肪酸(PLFA)方法研究进展［J］.山西农业科学, 2011,39(9):1020-1024.
【6】张秋芳,刘波,林营志,等.土壤微生物群落磷脂脂肪酸PLFA生物标记多样性［J］.生态学报, 2009,29(8):4127-4137.
【7】林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能［J］.土壤学报, 2008,45(5):892-900.
【8】BUYER J S. Rapid sample processing and fast gas chromatography for identification of bacteria by fatty acid analysis［J］. Journal of Microbiological Methods, 2002,51(2):209-215.
【9】吴愉萍,徐建明,汪海珍,等.Sherlock MIS系统应用于土壤细菌鉴定的研究［J］.土壤学报, 2006,43(4):642-647.
【10】BYSS M, TRISKA J, ELHOTTOVA D. GC-MS-MS analysis of bacterial fatty acids in heavily creosote-contaminated soil samples［J］. Analytical and Bioanalytical Chemistry, 2007,387(4):1573-1577.
【11】XUE Kai, LUO Hai-feng, QI Hong-yan, et al. Changes in soil microbial community structure associated with two types of genetically engineered plants analyzing by PLFA［J］. Journal of Environmental Sciences, 2005,17(1):130-134.
【12】FANG Jia-song, BARCELONA M J, ALVAREZ P J. A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification［J］. Organic Geochemistry, 2000,31(4):881-887.
【13】BOSELLI E, PACETTI D, CURZI F, et al. Determination of phospholipid molecular species in pork meat by high performance liquid chromatography-tandem mass spectrometry and evaporative light scattering detection［J］. Meat Science, 2008,78(3):305-313.
【14】CALDERON-SANTIAGO M, PRIEGO-CAPOTE F, GALACHE-OSUNA J G, et al. Analysis of serum phospholipid profiles by liquid chromatography-tandem mass spectrometry in high resolution mode for evaluation of atherosclerotic patients［J］. Journal of Chromatography A, 2014,1371(10):154-162.
【15】李范,周星梅,蒋海波,等.磷脂脂肪酸分析中内标物的使用及数据处理建模［J］.微生物学通报, 2014,41(12):2574-2581.
【16】祝伟霞,杨冀州,王彩娟,等.毛细管气相色谱法测定食用油中15种反式脂肪酸的含量［J］.理化检验-化学分册, 2015,51(3):375-380.
【17】SALOMONOVA S, LAMACOVA J, RULID M, et al. Determination of phospholipid fatty acids in sediments［J］. Facultas Rerum Naturalium, 2003,42:39-49.
【18】AZEVEDO A F D, LISBOA DUTRA J L D, SANTOS M L B, et al. Fatty acid profiles in Leishmania spp. isolates with natural resistance to nitric oxide and trivalent antimony［J］. Parasitology Research, 2014,113(1):19-27.
【19】任慧琴,赵念席,陈磊,等.土壤微生物群落结构分析中磷脂脂肪酸法和温和碱性甲酯化法的比较及定量优化［J］.环境化学, 2014,33(5):760-764.
【20】SCHUTTER M E, DICK R P. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities［J］. Soil Science Society of America Journal, 2000,64(5):1659-1668.
【2】KAUR A, CHAUDHARY A, KAUR A, et al. Phospholipid fatty acid-A bioindicator of environment monitoring and assessment in soil ecosystem［J］. Current Science, 2005,89(7):1103-1112.
【3】BUYER J S, SASSER M. High throughput phospholipid fatty acid analysis of soils［J］. Applied Soil Ecology, 2012,61(S1):127-130.
【4】齐鸿雁,薛凯,张洪勋.磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用［J］.生态学报, 2003,23(8):1576-1582.
【5】张瑞娟,李华,林勤保,等.土壤微生物群落表征中磷脂脂肪酸(PLFA)方法研究进展［J］.山西农业科学, 2011,39(9):1020-1024.
【6】张秋芳,刘波,林营志,等.土壤微生物群落磷脂脂肪酸PLFA生物标记多样性［J］.生态学报, 2009,29(8):4127-4137.
【7】林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能［J］.土壤学报, 2008,45(5):892-900.
【8】BUYER J S. Rapid sample processing and fast gas chromatography for identification of bacteria by fatty acid analysis［J］. Journal of Microbiological Methods, 2002,51(2):209-215.
【9】吴愉萍,徐建明,汪海珍,等.Sherlock MIS系统应用于土壤细菌鉴定的研究［J］.土壤学报, 2006,43(4):642-647.
【10】BYSS M, TRISKA J, ELHOTTOVA D. GC-MS-MS analysis of bacterial fatty acids in heavily creosote-contaminated soil samples［J］. Analytical and Bioanalytical Chemistry, 2007,387(4):1573-1577.
【11】XUE Kai, LUO Hai-feng, QI Hong-yan, et al. Changes in soil microbial community structure associated with two types of genetically engineered plants analyzing by PLFA［J］. Journal of Environmental Sciences, 2005,17(1):130-134.
【12】FANG Jia-song, BARCELONA M J, ALVAREZ P J. A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification［J］. Organic Geochemistry, 2000,31(4):881-887.
【13】BOSELLI E, PACETTI D, CURZI F, et al. Determination of phospholipid molecular species in pork meat by high performance liquid chromatography-tandem mass spectrometry and evaporative light scattering detection［J］. Meat Science, 2008,78(3):305-313.
【14】CALDERON-SANTIAGO M, PRIEGO-CAPOTE F, GALACHE-OSUNA J G, et al. Analysis of serum phospholipid profiles by liquid chromatography-tandem mass spectrometry in high resolution mode for evaluation of atherosclerotic patients［J］. Journal of Chromatography A, 2014,1371(10):154-162.
【15】李范,周星梅,蒋海波,等.磷脂脂肪酸分析中内标物的使用及数据处理建模［J］.微生物学通报, 2014,41(12):2574-2581.
【16】祝伟霞,杨冀州,王彩娟,等.毛细管气相色谱法测定食用油中15种反式脂肪酸的含量［J］.理化检验-化学分册, 2015,51(3):375-380.
【17】SALOMONOVA S, LAMACOVA J, RULID M, et al. Determination of phospholipid fatty acids in sediments［J］. Facultas Rerum Naturalium, 2003,42:39-49.
【18】AZEVEDO A F D, LISBOA DUTRA J L D, SANTOS M L B, et al. Fatty acid profiles in Leishmania spp. isolates with natural resistance to nitric oxide and trivalent antimony［J］. Parasitology Research, 2014,113(1):19-27.
【19】任慧琴,赵念席,陈磊,等.土壤微生物群落结构分析中磷脂脂肪酸法和温和碱性甲酯化法的比较及定量优化［J］.环境化学, 2014,33(5):760-764.
【20】SCHUTTER M E, DICK R P. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities［J］. Soil Science Society of America Journal, 2000,64(5):1659-1668.
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