十二烷基硫酸钠增敏荧光光谱法测定盐酸小檗碱
Determination of Berberine Hydrochloride by Fluorescent Spectrometry with Sodium Dodecyl Sulfate as Sensitizer
摘 要
基于十二烷基硫酸钠(SDS)对盐酸小檗碱荧光有显著增敏作用,建立了测定盐酸小檗碱的荧光光谱法。在pH 7.0的Tris-HCl缓冲溶液中,盐酸小檗碱与0.01 mol·L-1的SDS溶液反应15 min后,体系在530 nm处的荧光强度达到最大。盐酸小檗碱的浓度在1.0~10 mg·L-1范围内与体系荧光强度呈线性关系,检出限(3s/k)为0.23 mg·L-1。方法应用于制剂中盐酸小檗碱含量的测定,结果与药典法测定结果一致。加标回收率在94.9%~106%之间,测定值的相对标准偏差(n=6)在1.9%~3.7%之间。
盐酸小檗碱
十二烷基硫酸钠
增敏
Fluorescent spectrometry
Berberine hydrochloride
Sodium dodecyl sulfate
Sensitization
Abstract
A method of fluorescent spectrometry was developed for the determination of berberine hydrochloride, based on the sensitization effect of sodium dodecyl sulfate (SDS) on the fluorescence of berberine hydrochloride. In the buffer solution of Tris-HCl with pH of 7.0, berberine hydrochloride reacted with SDS solution (0.01 mol·L-1) for 15 min and the system displayed the maximum fluorenscence intensity at 530 nm. A linear relationship was found between the fluorescence intensity and the concentration of berberine hydrochloride in the range of 1.0-10 mg·L-1, with detection limit (3s/k) of 0.23 mg·L-1. The method was applied to the determination of berberine hydrochloride in pharmaceutical preparations, giving results consistent with that obtained by the pharmaceutical law. Recovery rates obtained by standard addition method were in the range of 94.9%-106% and RSDs (n=6) were in the range of 1.9%-3.7%.
中图分类号 O657.3 DOI 10.11973/lhjy-hx201510007
所属栏目 工作简报
基金项目 陕西省科技研究发展计划项目(2014JM2049);咸阳师范学院专项科研基金项目(14XSYK016)
收稿日期 2014/10/25
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备注郭颖(1978-),女,陕西咸阳人,讲师,博士,主要研究方向为发光分析。
引用该论文: GUO Ying,LI Wu-wu,LIU Yang,YANG Lian-li,LIU Jing. Determination of Berberine Hydrochloride by Fluorescent Spectrometry with Sodium Dodecyl Sulfate as Sensitizer[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(10): 1382～1385
郭颖,李午戊,刘洋,杨连利,刘静. 十二烷基硫酸钠增敏荧光光谱法测定盐酸小檗碱[J]. 理化检验－化学分册, 2015, 51(10): 1382～1385
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【6】李先文,黄强.小聚碱敏感场效应晶体管的研制与应用［J］.分析化学, 1997,25(11):1297-1299.
【7】李丽清,黄薇,丰满良,等.流动注射化学发光法测定小檗碱［J］.山东农业大学学报, 2002,33(3):365-367.
【8】迟家平,韩守智,薛守文,等.番泻叶口服液的制备与临床应用［J］.中成药, 1991(13):17-20.
【9】彭敬东,刘绍璞,刘忠芳,等.氯金酸-小檗碱离子缔合物体系的共振瑞利散射光谱研究及其分析应用［J］.化学学报, 2005,63(1):745-751.
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【11】SEEDHER N, AGARWAL P. Complexation of fluoroquinolone antibiotics with human serum albumin: a fluorescence quenching study［J］. Journal of Luminescence, 2010,130:1841-1848.
【12】GUO Dong-sheng, YUAN Xiao-ying, WU Jian-bing. Influence of Cr(Ⅲ) and Cr(Ⅵ) on the interaction between sparfloxacin and calf thymus DNA［J］. Journal of Inorganic Biochemistry, 2007,101(4):644-648.
【13】那佳,侯明.CdTe量子点的合成及作为离子荧光探针的应用［J］.桂林理工大学学报, 2010,30(1):133-136.
【14】SINGH H N, HINE W L. Micellar enhanced spectrofluorimetric methods: application to the determination of pyrene［J］. Analytical Letters, 1982,15(3):221-243.
【15】LIU Jing-fu, JONSSON J A, JIANG Gui-bin. Application of ionic liquids in analytical chemistry［J］. TrAC Trends in Analytical Chemistry, 2005,24(1):20-27.
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