高效液相色谱法测定地骨皮中18种氨基酸
HPLC Determination of 18 Amino Acids in Cortex Lycii
摘 要
采用高效液相色谱法测定地骨皮中18种氨基酸的含量。地骨皮样品用10-乙基吖啶酮-2-磺酰氯为柱前衍生试剂进行衍生化。以Akasil-C18色谱柱为固定相,用乙腈(5+95)溶液和乙腈(95+5)溶液以不同比例混合的溶液为流动相进行梯度洗脱,用荧光检测器测定。18种氨基酸的线性范围均为32 fmol~100 pmol,检出限(3S/N)在2.06~8.65 fmol之间。加标回收率在96.4%~107%之间,测定值的相对标准偏差(n=6)在均小于4.0%。
氨基酸
地骨皮
HPLC
Amino acids
Cortex lycii
Abstract
HPLC was applied to the determination of 18 amino acids in cortex lycii. The sample of cortex lycii was derived with 10-ethyl-acridine-2-sulfonyl chloride as precolumn derivatization reagent. Akasil-C18 chromatographic column was used as stationary phase with mixtures of acetonitile (5+95) solution and acetonitile (95+5) solution with different mixing ratios as mobile phase in the gradient elution, and determined with fluorescence detector. Linearity ranges of the 18 amino acides were found in the same range of 32 fmol-100 pmol with detection limits (3S/N) in the range of 2.06-8.65 fmol. Values of recovery found by standard addition method were in the range of 96.4%-107%, and values of RSD′s (n=6) less than 4.0%.
中图分类号 O652.63 DOI 10.11973/lhjy-hx201510022
所属栏目 工作简报
基金项目
收稿日期 2014/8/19
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联系人作者陈向明(xmch913@163.com)
备注陈向明(1978-),男,山东招远人,博士研究生,主要从事植物中活性成分分析。
引用该论文: CHEN Xiang-ming,SUO You-rui. HPLC Determination of 18 Amino Acids in Cortex Lycii[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(10): 1444～1447
陈向明,索有瑞. 高效液相色谱法测定地骨皮中18种氨基酸[J]. 理化检验－化学分册, 2015, 51(10): 1444～1447
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参考文献
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【5】陈飒飒,王钧,段世明.邻苯二甲醛柱前衍生反相高效液相色谱法测定氨基酸类神经递质［J］.理化检验-化学分册, 2003,39(5):283-285.
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【8】CHEN Guang, LI Jun, SUN Zhi-wei, et al. Rapid and sensitive ultrasonic-assisted derivatisation microextraction (UDME) technique for bitter taste-free amino acids (FAA) study by HPLC-FLD ［J］. Food Chemistry, 2014,143:97-105.
【2】魏智清,于洪川,樊瑞军.地骨皮降血糖有效成分的初步研究［J］.时珍国医国药, 2009,20(4):848-850.
【3】宁娜,韩建军.地骨皮的化学成分与药理作用［J］.现代药物与临床, 2010,25(3):172-176.
【4】魏秀丽,梁敬钰.地骨皮化学成分的研究［J］.中国药科大学学报, 2002,33(4):271-273.
【5】陈飒飒,王钧,段世明.邻苯二甲醛柱前衍生反相高效液相色谱法测定氨基酸类神经递质［J］.理化检验-化学分册, 2003,39(5):283-285.
【6】LOZANOV V, BENKOVA B, MATEVA L, et al. Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile［J］. Journal of Chromatography B, 2007,860(1):92-97.
【7】FVRST P, POLLACK L, GRASER T A, et al. Appraisal of four pre-column derivatization methods for the high-performance liquid chromatographic determination of free amino acids in biological materials［J］. J Chromatogr A, 1990,499:557-569.
【8】CHEN Guang, LI Jun, SUN Zhi-wei, et al. Rapid and sensitive ultrasonic-assisted derivatisation microextraction (UDME) technique for bitter taste-free amino acids (FAA) study by HPLC-FLD ［J］. Food Chemistry, 2014,143:97-105.
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