在弱酸性介质中,达旦黄(TY) 和氨基糖苷类抗生素(AGs)本身的共振瑞利散射(RRS)均很微弱.但两者相互作用形成离子缔合物时,溶液的RRS显著增强,并产生了新的RRS光谱.不同AGs的反应产物具有相似的光谱特征,其RRS峰位于278和469 nm;与此同时,它的倍频散射(FDS)也明显增强,并且最大FDS峰位于392 nm处,但二级散射(SOS)变化不明显.RRS较FDS具有较高的灵敏度,而且在一定范围内,AGs的浓度与散射强度(ΔIRRS)成正比.对于不同抗生素的检出限在17.2-23.3 μg·L-1之间,用于氨基糖苷类注射液和血清样品中AGs的测定,测得结果的RSD(n=5)值均小于3.5%,回收率在96.3%-97.2%之间.

Through the interaction of any of the AGs i.e.the kanamycin tobramycin,or gentamycin,with titan yellow in a weakly acidic solution,low intensity of resonance rayleigh scattering spectra (RRS) given by the reactants individually was found remarkably enhanced due to the formation of an ion-association complex,which gives a new RRS spectrum.The ion association complexes formed by different aminoglycosidic antibiotics showed similar spectral characteristics,and all their RRS spectra peaks are located at 278 nm and 469 nm.Simultaneously,the frequency doubling scattering (FDS) intensity were also raised distinctly.The peaks of FDS spectra are all located at 392 nm.But no obvious change of the 2nd order scattering (SOS) was observed.Linear relationship was found between the intensity of RRS (ΔIRRS) and the concentration of aminoglycosidic antibiotics in the range from 0-5.0 μg per 10 mL of solution,but with different detection limits (3σ) from 17.2-23.3 μg·L-1.Results of test of interfering substances showed that relatively good selectivity was achieved.Based on the above findings,a RRS spectrometric method for the determination of AGs was proposed,and in its application to the analysis of AGs injection and of human blood for AGs,RSD′s (n=5) obtained were less than 3.5%,and recoveries were in the range of 96.3%-97.2%.