A HIGHLY SENSITIVE PIEZO-ELECTRIC-IMMUNOSENSOR APPLIED TO THE DETERMINATION OF HDL
摘 要
采用自组装单层膜(SAMs)技术在压电石英晶体的金电极表面组装巯基丙酸SAMs,以盐酸1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)作偶联剂,以共价键合方式固定高密度脂蛋白抗体,研制成一种高灵敏的压电免疫传感器用于检测人血清中低含量的高密度脂蛋白.利用压电装置的实时监测功能,考察了巯基丙酸在金电极表面的自组装成膜过程与机制;研究了晶振固定抗体及其反应结合抗原的液相振荡行为和传感特性.传感器采用初始速率法测定高密度脂蛋白的线性浓度范围为1.63-18.8 mg·L-1,检出限为0.82 mg·L-1.
Abstract
It was reported in this paper that a highly sensitive piezo-immunosensor for using in the determination of medium or low contents of HDL in human sera was fabricated.In the fabrication,a monolayer of mercaptopropionic acid (MPA) was assembled on the surface of gold electrode of piezo-electric crystal by the self-assembled monlayer membrane (SAM) technique,during which 1-ethyl-3-(3-dimethylaminopropyl)-carbon diimine and N-hydroxy succinimide were used as coupling reagents and the HDL antibody was bound onto it by covalency.By the initial rate method,the linearity obtained by using this sensor in the determination of HDL was in the range of 1.63-18.8 mg·L-1 with a detection limit of 0.82 mg·L-1.Based on the real-time inspection function of the piezoelectric installation,the process and mechanism of the course of SAM of MPA on the surface of the gold electrode was investigated and immobilization of antibody by crystal oscillation,the behavior of liquid phase oscillation of the reaction of binding with antigens and the sensor characteristics were also studied.
中图分类号 O657.1
所属栏目 试验与研究
基金项目 国家自然科学基金资助课题(No.20075006,20205004)
收稿日期 2005/5/23
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备注李丹(1973-),女,湖南武冈人,硕士,主要从事分析测试及传感器的研究.
引用该论文: LI Dan,WANG Hua,WU Zhao-yang,SHEN Guo-li,YU Ru-qin. A HIGHLY SENSITIVE PIEZO-ELECTRIC-IMMUNOSENSOR APPLIED TO THE DETERMINATION OF HDL[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(4): 245~248
李丹,王桦,吴朝阳,沈国励,俞汝勤. 一种高灵敏的压电免疫传感器用于高密度脂蛋白的测定[J]. 理化检验-化学分册, 2006, 42(4): 245~248
被引情况:
【1】高先娟,王怀生,张玉军, "二乙腈基二硫纶自组装膜修饰金电极对多巴胺的电化学催化作用",理化检验-化学分册 50, 467-470(2014)
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参考文献
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【8】高志贤,张 超,陶桂全,等.压电免疫传感器抗体固定化方法研究[J].中华微生物和免疫学杂志,1996,16(3):191-195.
【9】Poirier G E,Pylant E D.The Self-Assembly Mechanism of Alkanethiols on Au(Ⅲ) [J].Science,1996,272:1145-1148.
【10】Ulman A.Formation and Structure of Self-Assembled Monolayers[J].Chem Rev,1996,96:1533-1554.
【11】Gauthier S,Aime J P,Bouhacina T,et al.Study of Grafted Silane Molecules on Silica Surface with an Atomic Force Microscope[J].Langmuir,1996,12:5126-5137.
【12】Megovern M E,Kallury K M R,Thomposon M.Role of Solvent on the Silanization of Glass with Octadecyltrichlorosilane[J].Langmuir,1994,10:3607-3614.
【13】Gao W,Reven L.Solid-State NMR Studies of Self-Assembled Monolayers[J].Langmuir,1995,11:1860-1863.
【14】Silberzan P,Leger L,Ausserre D,et al.Silanation of Silica Surfaces.A New Method of Constructing Pure or Mixed Monolayers[J].Langmuir,1991,7:1647-1651.
【15】Nakagawa T,Soga M.Contact Angle and Atomic Force Microscopy Study of Reactions of n-Alkyltrichlorosilanes with Muscovite Micas Exposed to Water Vapor Plasmas with Various Power Densities[J].Japnese Journal of Applled.Physics,1997,36:6915-6921.
【16】徐国华.OTS自组装单分子膜在玻璃表面形成过程的AFM研究[J].高等学校化学学报,2000,21(8):1257-1260.
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