CAPILLARY ELECTROPHORETIC DETERMINATION OF HUANGQIN GLUCOSIDE AND CHLOROGENIC ACID IN QINKAILING ORAL LIQUOR AND YINHUANG TABLETS
摘 要
通过系统优化缓冲溶液pH、工作电压和进样时间等因素的影响建立了毛细管电泳法分析清开灵口服液和银黄片中黄芩甙和绿原酸的方法.黄芩甙和绿原酸的峰高和浓度分别在006-1.20和0.02-0.50 g·L-1浓度范围内呈良好线性.基于迁移时间和峰高的相对标准偏差黄芩甙为1.60%和2.63%;绿原酸为1.48%和2.65%.根据3倍噪音法得出黄芩甙和绿原酸的检出限分别为0.020 g·L-1和0.004 g·L-1,实际样品分析的相对标准偏差均小于4%,利用银黄片做加标回收试验,黄芩甙和绿原酸的回收率分别为97.6%和97.3%.
Abstract
Influential factors (including the pH and concentration of buffer solution,the working voltage and the times of sample-introduction) of the capillary electrophoretic determination of baicalin and chlorogenic acid in “Qinkailing oral liquid” and “Yinhuang” tablets were studied and optimized.Linear relationships between peak heights and concentrations for baicalin and chlorogenic acid were obtained in the ranges of 0.06-1.20 g·L-1 and 0.02-0.50 g·L-1 respectively.RSD′s found for baicalin were 1.60% (based on migration time) and 2.63% (based on peak height),and for chlorogenic acid were 1.48% (based on migration time) and 2.65% (based on peak height).Detection limit (S/N=3) obtained for baicalin was 0.020 g·L-1 and for chlorogenic acid was 0.004 g·L-1.In the application of the proposed method to the analysis of two drug samples,RSD′s of less than 4% were obtained.Recovery test was made by standard addition method based on “Yinhuang” tablet sample,and values of 97.6% (for baicalin) and 97.3% (for chlorogenic acid) were obtained.
中图分类号 O657.8
所属栏目 工作简报
基金项目 国家自然科学基金(20175025,29875027,20235010)资助项目
收稿日期 2004/6/21
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备注吕元琦(1974-),男,山东陵县人,博士,从事高效毛细管电泳和等离子体质谱研究.
引用该论文: LU Yuan-qi,WU Chun-hua,YUAN Zhuo-bin. CAPILLARY ELECTROPHORETIC DETERMINATION OF HUANGQIN GLUCOSIDE AND CHLOROGENIC ACID IN QINKAILING ORAL LIQUOR AND YINHUANG TABLETS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(5): 337~338
吕元琦,邬春华,袁倬斌. 毛细管电泳法测定清开灵口服液和银黄片中的黄芩甙和绿原酸[J]. 理化检验-化学分册, 2006, 42(5): 337~338
被引情况:
【1】顾宇翔,周泽琳,杨轶眉,冯俊,汪霄峰, "高效液相色谱法测定化妆品中黄芩甙",理化检验-化学分册 48, 442-444(2012)
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参考文献
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【3】Cao Y,Chu Q,Fang Y,et al.Analysis of Flavonoids in Ginkgo Biloba L.and its Phytopharmaceuticals by Capillary Electrophoresis with Electrochemical Detection[J].Anal Bioanal Chem,2002,374(2):294.
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