氨苄青霉素降解产物荧光光度测定的研究
STUDY ON THE FLUOROPHOTOMETRY OF THE DEGRADATON PRODUCT OF AMPICILLIN
摘 要
在氨水-NH4Cl介质(pH 9.0)和沸水浴加热下,铜离子对氨苄青霉素的降解反应具有催化作用,其降解产物可产生荧光.据此建立了测定氨苄青霉素的方法.氨苄青霉素的浓度在1.5×10-7-3.0×10-6mol·L-1范围内与荧光强度呈线性关系,检出限为4.0×10-8mol·L-1.可用于氨苄青霉素胶囊的分析及尿液中氨苄青霉素的测定,平均回收率为95.3%-101.4%.研究表明,反应的活化能为34.88 kJ·mol-1,100 ℃速率常数为4.68 h-1.
氨苄青霉素
药物分析
Fluorophotometry
Ampicillin
Pharmaceutical analysis
Abstract
A fluorophotometric method for the determination of ampicillin was established in this paper.The method was based on that the ampicillin was degradated under conditions of pH 9.0 in an ammoniacal buffer medium and of the presence of Cu(Ⅱ) as catalyst and heating with boiling water bath,and the fluorescence intensity of the degradation product was determined fluorophotometrically.The activation energy of 34.88 kJ·mol-1 and the reaction rate constant of 4.68 h-1 were found.A linear relationship between the fluorescence intensity and ampicillin concentration in range of 1.5×10-7-3.0×10-6mol·L-1 was obtained.Detection limit was 4.0×10-8mol·L-1 and recoveries were in the range 95.3%-101.4%.The method has been applied to the determination of ampicillin in capsules and urine samples.
中图分类号 O657.31
所属栏目 工作简报
基金项目 广东省教育厅自然科学研究项目(0164)
收稿日期 2004/9/11
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备注任乃林(1962-),男,陕西岐山县人,副教授,主要从事药物分析研究工作.
引用该论文: REN Nai-lin,CHEN Shao-jin,LI Guo-hong. STUDY ON THE FLUOROPHOTOMETRY OF THE DEGRADATON PRODUCT OF AMPICILLIN[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(5): 342～343
任乃林,陈少瑾,李国红. 氨苄青霉素降解产物荧光光度测定的研究[J]. 理化检验－化学分册, 2006, 42(5): 342～343
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参考文献
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【3】李华侃,赵凤林,童沈阳.荷移反应用于氨苄青霉素的测定[J].分析化学,1997,25(1):46-48.
【4】Forsman U.Cathodic Stripping Voltammetric Determination of Trace amounts of Penicillins[J].Anal Chim Acta,1983,146:71-86.
【5】张玉忠,袁倬斌.氨苄青霉素降解产物在汞膜电极上的伏安法研究[J].分析试验室,2001,20(4):16-18.
【6】欧阳耀国,蔡维平,罗万红,等.光化学荧光分析法测定氨苄青霉素[J].厦门大学学报(自然科学版),1994,33(3):418-420.
【7】何锡文,唐 波,沈含熙.头孢氨苄降解产物荧光性质的研究及分析应用[J].高等学校化学学报,1995,169(1):26-30.
【8】Navarro P G,Bekkouri A E,Reinoso E R.Spectrofluorimetric Study of the Degradation of α-Amino β-lactam Antibiotics Catalysed by Metal ions in Methanol[J].The Analyst,1998,123:2263-2266.
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