毛细管电泳紫外检测聚谷氨酸发酵液中蔗糖浓度
DETERMINATION OF SUCROSE IN POLY-γ-GLUTAMIC ACID FERMENTATION LIQUOR BY CAPILLARY ELECTROPHORESIS WITH UV DETECTION
摘 要
建立了测定发酵液中蔗糖浓度的新方法——毛细管电泳紫外法.考察了该方法中背景缓冲液的选择、分离电压、温度等因素对样品响应值和迁移时间的影响,获得了优化的电泳工作条件.最佳的操作条件为：以30 mmol·L-1、pH 11.0的对氨基水杨酸作为背景缓冲液,分离电压15 kV,操作温度25 ℃.在此条件下,电泳分离在6.66 min内完成,标准曲线的线性范围为0078-20 g·L-1、r为0.999 8、检出限为0.035 g·L-1.应用该方法测定聚谷氨酸发酵过程中不同生长时期蔗糖浓度和消耗曲线.
紫外检测法
蔗糖
γ-聚谷氨酸
Capillary electrophoresis
UV detection
Sucrose
Poly γ-glutamic acidγ-
Abstract
A method of capillary electrophoresis with UV detection was established for the determination of sucrose in poly-γ-glutamic acid (γ-PGA) fermentation liquor.Effects of running buffer and its concentration,separation voltage and temperature on the retention time and signal response were investigated.Optimized working conditions were described as follows: aminosalicylic acid (30 mmol·L-1 pH 11.0) as the running buffer,separation voltage: 15 kV,operating temperature: 25 ℃.Electrophoresis separation was performed within 6.66 min.Linearity of the calibration curve was in range of 0.078-20 g·L-1 with a detection limit of 0.035 g·L-1 and r 0.999 8.The method has been applied to the mapping of the sucrose consumption curve and determination of concentration of sucrose at different growing stage during the course of fermentation of γ-PGA.
中图分类号 O657.8
所属栏目 试验与研究
基金项目
收稿日期 2005/7/5
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备注刘岑(1979-),女,湖北人,硕士,主要研究方向为枯草芽孢杆菌发酵生产聚谷氨酸.
引用该论文: LIU Cen,SHI Feng,XU Zhi-nan,CEN Pei-lin. DETERMINATION OF SUCROSE IN POLY-γ-GLUTAMIC ACID FERMENTATION LIQUOR BY CAPILLARY ELECTROPHORESIS WITH UV DETECTION[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(6): 417～419
刘岑,石峰,徐志南,岑沛霖. 毛细管电泳紫外检测聚谷氨酸发酵液中蔗糖浓度[J]. 理化检验－化学分册, 2006, 42(6): 417～419
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参考文献
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【2】桑莉,徐虹,李 晖,等.γ-聚谷氨酸产生菌的筛选及发酵条件[J].过程工程学报,2004,4(5):462-466.
【3】Xu Hong,Jiang Min,Li Hui,et al.Efficient Production of Poly (γ-Glutamic Acid)by Newly Isolated Bacillus Subtilis NX-2[J].Process Biocemistry,2005,40:519-523.
【4】李利军,孔红星,陆丹梅.蒽酮-硫酸法快速测定蔗糖的研究及应用[J].分析检测,2003,24(10):145-149.
【5】章朝晖,蔗糖化学衍生物[J].精细石油化工进展,2000,11(6):8-13.
【6】李利军,孔红星,何云,等.蔗糖中蔗糖含量的分光光度法测定[J].分析测试学报,2003,22(4):51-54.
【7】任吉存,李斌成,邓延倬,等.毛细管电泳-激光干涉检测法测定饮料中蔗糖含量[J].分析化学,1995,23(9):1040-1042.
【8】余兆楼,常理文,胡喜章,等.单糖的高效毛细管电泳间接紫外检测法研究[J].分析化学,1994,22(8):755-758.
【9】陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2001.134-145.
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