膜载体酶标记dip-stick快速检测西维因的痕量残留
RAPID DETECTION OF INSECTICIDE CARBARYL WITH MEMBRANE-CARRIER DIP-STICK ENZYME IMMUNOASSAY
摘 要
利用直接竞争酶联免疫吸附分析技术研发了一种快速、灵敏的膜载体酶标记dip-stick农残西维因检测方法.该方法以带正电尼龙膜(Millipore)为固相载体,将西维因抗体包被于膜上,包被有抗体的膜插入含有西维因和西维因酶标抗原的混合液中竞争反应10 min后直接目视判断分析结果.该方法的整个检测过程只需15 min,对西维因的检出限可达到10 μg·L-1,且稳定性较好.通过与高效液相色谱法(HPLC)比较,验证了该检测方法的有效性,可作为品质控制和快速定性检测的一种有效工具.
西维因
带正电尼龙膜
定性检测
Dipstick immunoassay
Carbaryl
Positively charged Nylon membrane
Detection
Abstract
A rapid and sensitive dipstick immunoassay for detection of carbaryl was described.The assaying was based on the competitive principle and an anti-carbaryl polyclonal antibody immobilized on positively charged Nylon membrane was used.A strip of the nembrane coated with antibody was immersed into the solution mixture of antigen and antigen-HRP for 10 min.The assay was completed within 15 min and the limit of detection was 10 μg·L-1.Validity of the dipstick assaying was verified by HPLC and it was proved to be an efficient method for quality control.
中图分类号 X836
所属栏目 试验与研究
基金项目
收稿日期 2005/9/26
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备注张灿(1979-),女,湖南人,硕士研究生,研究方向为农药残留快速检测.
引用该论文: ZHANG Can,WANG Jun-ping,YU Chun-di,WANG Shuo. RAPID DETECTION OF INSECTICIDE CARBARYL WITH MEMBRANE-CARRIER DIP-STICK ENZYME IMMUNOASSAY[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(6): 420～422
张灿,王俊平,于春娣,王硕. 膜载体酶标记dip-stick快速检测西维因的痕量残留[J]. 理化检验－化学分册, 2006, 42(6): 420～422
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参考文献
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【2】Cho Y A,Kim Y J,Hammock B D,et al.Development of a Microtiter Plate ELISA and a Dipstick ELISA for the Determination of the Organophosphorus Insecticide Fenthion[J].J Agric Food Chem,2003,51(27):7854-7860.
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【5】Stephan O,Mller N,Lehmann S,et al.Development and Validation of Two Dipstick Type Immunoassays for Determination of Trace Amounts of Peanut and Hazelnut in Processed Foods[J].Eur Food Res Technol,2002,215:431-436.
【6】Sager S D,Peteghem C V.Dipstick Enzyme Immunoassay to Detect Fusarium T-2 Toxin in Wheat[J].Applied and Environmental Microbiology,1996,6:1880-1884.
【7】Kristina E I,Arts J M,Peteghem C H Van.Development of a Fast and Simple Method for Determination of β-Agonists in Urine by Extraction on Empore Membranes and Detection by a Test Strip Immunoassay[J].J Agric Food Chem,1997,45:3129-3137.
【8】Wang S,Allan R D,Hill A S,et al.Rapid Enzyme Immunoassays for the Detection of Carbaryl and Methoprene in Grain[J].J Environ Science and Health,2002,B37:521-532.
【2】Cho Y A,Kim Y J,Hammock B D,et al.Development of a Microtiter Plate ELISA and a Dipstick ELISA for the Determination of the Organophosphorus Insecticide Fenthion[J].J Agric Food Chem,2003,51(27):7854-7860.
【3】Gabldon J A,Maquieira A,Puchades R.Rapid Method for On-Site Determination of Atrazine Residues in Water Samples[J].Intern J Environ Anal Chem,2002,82(3):133-144.
【4】Baumgartner S,Steiner I,Kloiber S,et al.Towards the Development of a Dipstick Immunoassay for the Detection of Trace Amount of Egg Proteins in food[J].Eur Food Res Technol,2002,214:168-170.
【5】Stephan O,Mller N,Lehmann S,et al.Development and Validation of Two Dipstick Type Immunoassays for Determination of Trace Amounts of Peanut and Hazelnut in Processed Foods[J].Eur Food Res Technol,2002,215:431-436.
【6】Sager S D,Peteghem C V.Dipstick Enzyme Immunoassay to Detect Fusarium T-2 Toxin in Wheat[J].Applied and Environmental Microbiology,1996,6:1880-1884.
【7】Kristina E I,Arts J M,Peteghem C H Van.Development of a Fast and Simple Method for Determination of β-Agonists in Urine by Extraction on Empore Membranes and Detection by a Test Strip Immunoassay[J].J Agric Food Chem,1997,45:3129-3137.
【8】Wang S,Allan R D,Hill A S,et al.Rapid Enzyme Immunoassays for the Detection of Carbaryl and Methoprene in Grain[J].J Environ Science and Health,2002,B37:521-532.
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