HPLC DETERMINATION OF BENZOYL PEROXIDE IN VERMICELLI
摘 要
利用高效液相色谱测定粉丝中过氧化苯甲酰,采用了以还原铁粉作为还原剂将过氧化苯甲酰还原成苯甲酸,0.02 mol·L-1氢氧化钠作为提取液,滤液经色谱分离后,在检测器230 nm下测定苯甲酸的含量.线性范围为1-50 mg·L-1(r=0.999 9),检出限为0.2 mg·L-1,RSD为1.49%-4.61%(n=6),回收率为88.0%-97.5%.
Abstract
An advance was made in the pretreatment of the powdered and homogeneized vermicelli sample by a reduction with 0.1 g of iron powder in 90 mL of 0.02 mol·L-1 NaOH solution to transform the benzoyl peroxide (BZPX) into benzoic acid (BZA).The acidity of the NaOH solution was neutralized to about pH 5 by adding 10 mL of a 0.02 mol·L-1 NH4OAc buffer solution (previously adjusted to pH 3.6 by glacial HOAC).The solution was filtered,separated on the Agilant Eclipse XDB-C18 column and finally measured at 230 nm with UV-detector.Range of linearity for the determination of benzoyl peroxide (in the form of benzoic acid) was found form 1-50 mg·L-1 (r=0.999 9) with a detection limit of 0.2 mg·L-1 (S/N=3).Test for precision and recovery gave values of RSD′s (n=6) in the range of 1.49%-4.61% and values of recoveries in the range from 88.0% to 97.5%.
中图分类号 O657.31
所属栏目 工作简报
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收稿日期 2004/6/28
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备注钟全斌(1955-),男,浙江人,高级工程师,主要研究方向为食品分析.
引用该论文: ZHONG Quan-bin,NING Xiao-jun,LI Zhao-jie,ZHANG Yan-qin. HPLC DETERMINATION OF BENZOYL PEROXIDE IN VERMICELLI[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(6): 435~436
钟全斌,宁啸骏,李兆阶,张燕琴. 高效液相色谱法测定粉丝中过氧化苯甲酰[J]. 理化检验-化学分册, 2006, 42(6): 435~436
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参考文献
【1】GB/T 18415-2001,小麦粉中过氧化苯甲酰的测定方法[S].
【2】GB/T 5009.29-2003,食品中山梨酸、苯甲酸的测定方法[S].
【3】王勤,张存玲,徐文杰.高效液相色谱测定面粉中过氧化苯甲酰[J].理化检验-化学分册,2002,38(7):361.
【4】GB2760-1996,食品添加剂使用卫生标准[S].
【2】GB/T 5009.29-2003,食品中山梨酸、苯甲酸的测定方法[S].
【3】王勤,张存玲,徐文杰.高效液相色谱测定面粉中过氧化苯甲酰[J].理化检验-化学分册,2002,38(7):361.
【4】GB2760-1996,食品添加剂使用卫生标准[S].
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