高效液相色谱电化学发光法检测抗坏血酸
DETERMINATION OF ASCORBIC ACID BY HPLC COMBINED WITH ELECTROCHEMILUMINESCENCE DETECTION
摘 要
基于在恒电流条件下电解产生不稳定化学发光试剂Mn(Ⅲ)能直接氧化抗坏血酸产生化学发光,将高效液相色谱法与电化学发光法相结合,建立了高效液相色谱电化学发光法测定抗坏血酸的检测方法.在优化的试验条件下,对啤酒、人体血清、尿液中抗坏血酸含量进行了分析,方法的检出限为3.0×10-5g·L-1, 相对标准偏差为2.2%(n=11,5.0×10-2g·L-1 VC),线性范围1×10-4-1×10-1g·L-1.
电化学发光
锰(Ⅲ)
抗坏血酸
High performance liquid chromatography
Electrochemiluminescence
Mn(Ⅲ)
Ascorbic acid
Abstract
A method of HPLC combined with electrochemiluminescence detection for the determination of ascorbic acid was established on the basis of that the ascorbic acid could be oxidized by Mn(Ⅲ) which was produced under conditions of constant current electrodecomposition,resulting a chemiluminescence.The proposed method has a detection limit of 3×10-5g·L-1.RSD of 2.2% (n=11,at concentration level of 5.0×10-2g·L-1) and linearity range from 1×10-4 to 1×10-1g·L-1.Ascorbic acid in several complex samples such as beer,serum and urine was determined under optimized conditions with satisfactory results.
中图分类号 O657.3
所属栏目 工作简报
基金项目
收稿日期 2004/9/15
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备注杨红兵(1968-),女,新疆人,副教授,硕士,分析化学专业.
引用该论文: YANG Hong-bing,ZHANG Cheng-xiao,HONG Cheng-ling. DETERMINATION OF ASCORBIC ACID BY HPLC COMBINED WITH ELECTROCHEMILUMINESCENCE DETECTION[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(7): 526～528
杨红兵,张成孝,洪成林. 高效液相色谱电化学发光法检测抗坏血酸[J]. 理化检验－化学分册, 2006, 42(7): 526～528
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参考文献
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【2】刘海玲,欧水平.阻抑动力学光度法测定抗坏血酸[J].分析化学,2002,30(1):122．
【3】Tabata M,Morita H.Spectrophotometric Determination of a Nanomolar Amount of Ascorbic Acid using its Catalytic Effect on Copper(Ⅱ) Porphyrin Formation[J].Talanta,1997,44(2):151-157．
【4】司文会.福林试剂光度法快速测定全血中抗坏血酸[J].理化检测-化学分册,2000,36(5):212-213．
【5】Koshiishi I,Imanari T.Measurement of Ascorbate and Dehydroascorbate Contents in Biological Fluids[J].Anal Chem,1997,69(2):216-220．
【6】李连朝,康靖全,杨德祥.用反相高效液相色谱法测定食物药物和生理体液中的维生素C[J].色谱,1986,4(6):334-338．
【7】张国峰,缪刚.高效液相色谱法测定芦丁片和维生素C的含量[J].色谱,1996,14(3):227-228．
【8】李 峰,朱果逸.铬(Ⅵ)-过氧化氢-鲁米诺化学发光法测定痕量抗坏血酸[J].分析化学,2002,30(5):580-582．
【9】李峰,张文艳,朱果逸.流动注射化学发光抑制法测定抗坏血酸[J].分析化学,2000,28(12):1523-1526．
【10】杜建修,李银环,吕九如.在线偶合化学发光法测定抗坏血酸[J].陕西师范大学学报,2000,28(4):81-83．
【11】Chen Xi,Sato Massanori.High-Performance Liquid Chromatographic Determination of Ascorbic Acid in Soft Drinks and Apple Juice Using Tris (2,2,-Bipyridine) Ruthenium(Ⅱ) Electrochemiluminescence[J].Anal Sci,1995,11(5):749-754．
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