单扫描极谱法测定复方青黛片中的吲哚醌
LINEAR SWEEPING POLAROGRAPHIC DETERMINATION OF ISATIN IN NATURAL INDIGO COMPOUND TABLETS
摘 要
应用单扫描极谱法(LSV)及循环伏安法(CV),在pH 9.8的氨性缓冲液中,对2,3-吲哚醌(ISA)的电化学行为进行了研究.在该缓冲液中ISA产生p1、p2两个还原峰,峰电位分别为-0.51 V和-0.59 V(vs.SCE).其中p1峰峰电流i″p值与ISA的浓度在2×10-6-2×10-4mol·L-1范围内呈线性关系(r=0.999 5).检出限为7×10-7mol·L-1,对浓度为4×10-5mol·L-1的ISA溶液进行11次平行测定,RSD为1.29%.所建立的方法已用于复方青黛片中ISA含量的测定.
循环伏安法
2,3-吲哚醌
复方青黛片
Linear sweeping polarographic
Cyclic voltammetry
Isatin
Natural Indigo compound tablet2,3-
Abstract
In an ammoniacal buffer medium of pH 9.8,the electro-chemical behavior of 2,3-isatin (ISA) was studied by linear sweeping voltammetry and cyclic voltammetry.Two reductive waves of ISA were observed with peak potentials,at -0.51 V and -0.59 V (vs.SCE).Linear relationship between the values of 2nd derivative of peak current (i″p) at Ep of -0.51 V and of concentration of ISA was obtained in the range of 2×10-6-2×10-4mol·L-1 (r=0.999 5).The detection limit was found to be 7×10-7mol·L-1 of ISA.Precision tested at the concentration level of 4×10-5mol·L-1 of ISA gave a value of RSD (n=11) of 1.29%.The proposed method has been used in the determination of ISA in natural Indigo compound tablets.
中图分类号 O657.12
所属栏目 工作简报
基金项目 陕西省教育厅专项科研基金资助项目(03JK216)
收稿日期 2005/7/20
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备注王福民(1964-),男,陕西蒲城人,副教授,硕士,研究方向为电分析化学.
引用该论文: WANG Fu-min. LINEAR SWEEPING POLAROGRAPHIC DETERMINATION OF ISATIN IN NATURAL INDIGO COMPOUND TABLETS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(7): 539～541
王福民. 单扫描极谱法测定复方青黛片中的吲哚醌[J]. 理化检验－化学分册, 2006, 42(7): 539～541
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参考文献
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【2】Hamaue N,Minami M,Kanamarn Y,et al.Endogenous Monoamine Oxidase (MAO) Inhibitor (Tribulin-Like Activity) in the Brain and Urine of Stroke-Prone SHR[J].Biog Amines,1992,8(6):401．
【3】McIntyre I M,Norman T R.Serotonergic Effects of Isatin,an Endogenous MAO Inhibitor Related to Tribulin[J].Neural Transm [Gen sect],1990,79(1-2):35．
【4】李清华.青黛的化学成分[J].植物学报,1987,29(1):67．
【5】Hamaue N,Yamazaki N,Minami M,et al.Determination of Isatin,an Endogenous Monoamine Oxidase Inhibitor,in Urine and Tissues of Rats by HPLC[J].Gen Pharmc,1989,30(3):387．
【6】Makiko K,Segawa M,Masatsuka R,et al.Fluorimetric Determination of Isatin in Human Urine and Serum by Liquid Chromatography Postcolumn Photoirradiation[J].Analyst,2001,126(1):33．
【7】李静,孙伟,李娅娟.尿中2,3-吲哚醌作为帕金森病预警标志物的研究[J].中国药学杂志,2004,39(10):776．
【8】李启隆.电分析化学[M].北京:北京师范大学出版社,1997:202-228．
【2】Hamaue N,Minami M,Kanamarn Y,et al.Endogenous Monoamine Oxidase (MAO) Inhibitor (Tribulin-Like Activity) in the Brain and Urine of Stroke-Prone SHR[J].Biog Amines,1992,8(6):401．
【3】McIntyre I M,Norman T R.Serotonergic Effects of Isatin,an Endogenous MAO Inhibitor Related to Tribulin[J].Neural Transm [Gen sect],1990,79(1-2):35．
【4】李清华.青黛的化学成分[J].植物学报,1987,29(1):67．
【5】Hamaue N,Yamazaki N,Minami M,et al.Determination of Isatin,an Endogenous Monoamine Oxidase Inhibitor,in Urine and Tissues of Rats by HPLC[J].Gen Pharmc,1989,30(3):387．
【6】Makiko K,Segawa M,Masatsuka R,et al.Fluorimetric Determination of Isatin in Human Urine and Serum by Liquid Chromatography Postcolumn Photoirradiation[J].Analyst,2001,126(1):33．
【7】李静,孙伟,李娅娟.尿中2,3-吲哚醌作为帕金森病预警标志物的研究[J].中国药学杂志,2004,39(10):776．
【8】李启隆.电分析化学[M].北京:北京师范大学出版社,1997:202-228．
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