微型多酚氧化酶电极的研制及其应用
THE FABRICATION AND APPLICATION OF THE POLYPHENOL OXIDASE MICROELECTRODE
摘 要
研制了一种微型多酚氧化酶修饰电极,研究了神经递质多巴胺(DA)在电极上的电化学行为.在pH 6.8的磷酸盐中,DA在生物传感器上的线性范围为2.0×10-6-1.0×10-3mol·L-1,检出限(3σ)为6.0×10-7mol·L-1.用这种传感器测定了多巴胺注射液中多巴胺的含量,测定结果的RSD值小于3%,回收率在95%-110%之间.
多酚氧化酶
微电极
Dopamine
Polyphenol oxidase
Microelectrode
Abstract
A microelectrode modified with polyphenol oxidase was fabricated and used as a biosensor.Electrochemical behavior of dopamine at the electrode was studied.In a phosphate buffer of pH 6.8,linear response of dopamine at the biosensor was obtained in the concentration range of 2.0×10-6-1.0×10-3mol·L-1.Detection limit (3σ) of 6.0×10-7mol·L-1was obtained.The biosensor has been applied to the determination of dopamine in medical injections,giving RSD′s less than 3%,and recoveries in the range from 95%-110%.
中图分类号 O657.1
所属栏目 工作简报
基金项目 湖南省教育厅资助项目(02B027)
收稿日期 2004/9/16
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备注邓培红(1975-),女,广西南宁人,硕士,讲师,主要从事生物电化学方面的研究.
引用该论文: DENG Pei-hong,ZHANG Jun,KUANG Yun-fei,LI Ju-nan. THE FABRICATION AND APPLICATION OF THE POLYPHENOL OXIDASE MICROELECTRODE[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(7): 562～564
邓培红,张军,匡云飞,黎拘难. 微型多酚氧化酶电极的研制及其应用[J]. 理化检验－化学分册, 2006, 42(7): 562～564
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参考文献
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【10】Tu Yi-Feng,Fu Zhi-Qiang,Chen Hong-Yuan.The Fabrication and Optimization of the Disposable Amperometric Biosensor[J].Sensor and Actuators B,2001,80:101-105．
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【3】田敏,董少俊.神经递质多巴胺在AQ修饰组合微电极上的伏安行为及其伏安溶出法测定[J].分析化学,1994,22(1):15-18．
【4】Chen Y,Tan T C.Dopamine Sensing and Selectivity of Nafion-Coated Plant Tissue Powder Sensors[J].Talanta,1995,42:1181-1188．
【5】Chen Y,Tan T C.Dopamine-Sensing Efficacy and Characteristics of Pretreated Plant Tissue Powder Sensors[J].Sensors and Actuators B,1995,28:39-48．
【6】Chen Hong-yuan,Yu Ai-min,Xu Dan-ke.Development of an Amperometric Detector for the Determination of Phenolic Compounds[J].Fresenius J Anal Chem,1997,359:542-545．
【7】孙元喜,冶保献,周性尧.聚中性红膜修饰电极上神经递质的电化学行为及应用[J].分析化学,1998,26(5):506-510．
【8】万其进,喻玖宏,王刚,等.聚茜素红膜修饰电极控制电位扫描法分别测定多巴胺和抗坏血酸[J].高等学校化学学报,2000,21:1651-1654．
【9】林祥钦,晋冠平,崔华.聚L-谷氨酸/石墨充蜡修饰电极测定多巴胺[J].分析化学,2002,30(3):271-275．
【10】Tu Yi-Feng,Fu Zhi-Qiang,Chen Hong-Yuan.The Fabrication and Optimization of the Disposable Amperometric Biosensor[J].Sensor and Actuators B,2001,80:101-105．
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