在盐酸介质中,以苯基荧光酮为显色剂,吐温-80为表面活性剂,明胶为稳定剂,用增溶胶束分光光度法测定微量锗.探讨了测定微量锗的最佳显色条件和光度测定条件.配合物的最大吸收位于510 nm波长处,锗含量在0.5-10 μg/50 mL范围内符合比耳定律,其摩尔吸光系数ε=1.18×105L·mol-1·cm-1,具有较高的灵敏度和选择性,用于人发中锗含量的测定,测定结果的RSD小于0.9%,加标回收率在95.9%-105.9%.

In a solution of 0.84 mol·L-1 HCl,a sensitive color reaction between Ge(Ⅳ) and the reaction system of phenylfluorone-Tween-80-gelatin was observed.The non-ionic surfactant,Tween-80 acted as a solubilizing and sensitizing agent,and the gelatin acted as a stabilizing agent in the reaction system.Absorption maxima of the colored coordination complex was found at 510 nm,with a molar absorptivity of 1.18×105L·mol-1·cm-1.Beer′s law was obeyed in the concentration range of 0.5-10 μg per 50 mL of solution.The interferences of some commonly encountered ions were tested,and the selectivity was satisfactory for its application to the determination of Ge(Ⅳ) in human hair samples,where the RSD′s obtained were less than 0.9%,and the recoveries found were in the range of 95.9% to 105.9%.