HPLC DETERMINATION OF GLYOXALIC ACID IN PRESENCE OF GLYCOLIC ACID AND OXALIC ACID
摘 要
采用反相高效液相色谱分离测定了乙醇酸和草酸共存下乙醛酸的含量.色谱柱为Hypersil ODS-C18柱(4.6 mm×100 mm,5 μm),流动相为乙腈-水,检测波长为210 nm.探讨了流动相的pH及其配比对分离度和灵敏度的影响.以峰面积对乙醛酸浓度(g·L-1)作图所得校正曲线的线性回归方程为Y=1.934×103 X+0.067×103(r=0.999 9),方法的检出限(2S/N)为5×10-7g·L-1.方法的测定回收率为98.1%-100.4%,相对标准偏差为0.4%.
Abstract
A simple and rapid reversed-phase high performance liquid chromatographic method was applied to the determination of the content of glyoxalic acid in the presence of glycolic acid and oxalic acid.Hypersil ODS C18 column,a mobile phase of acetonitrile-water mixed solution and UV detection at 210 nm were employed in the determination.The effects of pH and composition of the mobile phase on the sensitivity and separation of the method were studied.Linearity between the peak area and concentration of glyoxalic acid (g·L-1) was tested and the following regression equation was obtained Y=1.934×103X+0.067×103 (r=0.999 9).The detection limit (2S/N) was found to be 5×10-7g·L-1.Test for precision at the same concentration for 7 determinations gave a value of RSD of 0.4%.Recoveries obtained by the standard addition method were in the range of 98.1%-100.4%.
中图分类号 O657.72
所属栏目 工作简报
基金项目 上海市教委重点培育学科基金资助项目
收稿日期 2004/12/11
修改稿日期
网络出版日期
作者单位点击查看
备注卢立泓(1962-),女,上海市人,讲师,从事教学和色谱工作.
引用该论文: LU Li-hong,TAO Jian-wei,LU Qing-ning,ZHANG Han-zhi,TANG Yi-lan. HPLC DETERMINATION OF GLYOXALIC ACID IN PRESENCE OF GLYCOLIC ACID AND OXALIC ACID[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(8): 644~645
卢立泓,陶建伟,陆庆宁,张菡子,唐一兰. 反相高效液相色谱测定在乙醇酸和草酸存在下的乙醛酸含量[J]. 理化检验-化学分册, 2006, 42(8): 644~645
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】胡军,张新胜,吴明东,等.乙二醛阳极氧化制乙醛酸过程的化学分析方法[J].华东理工大学报,2001,27(1):34-37.
【2】范富龙,施耀曾,杨士英,等.在乙二醛等化合物存在下乙二醛测定方法研究[J].南京化学学报,1989,25(2):308-313.
【3】徐嘉凉,王诚瑜,汤晓东.双波长和三波长分光光度法同时测定乙醛酸和乙二醛[J].分析化学,1997,25(9):1086-1089.
【4】朱明华.仪器分析.第三版[M].北京:高等教育出版社,2000:13-90.
【5】刘庆彬,薄海静,郭秀斌,等.乙醛酸水溶液的高效液相色谱分析[J].分析化学,1998,26(1):119.
【6】施奈德 L R,格莱吉客 J L,柯克兰 J J.实用高效液相色谱法的建立[M].北京:科学出版社,1998:85-86.
【7】Bevilacqua A E,Califano A N.Determination of organic acids in dairy products by high performance liquid chromatograghy[J].Food Science,1989,54(4):1076-1077.
【2】范富龙,施耀曾,杨士英,等.在乙二醛等化合物存在下乙二醛测定方法研究[J].南京化学学报,1989,25(2):308-313.
【3】徐嘉凉,王诚瑜,汤晓东.双波长和三波长分光光度法同时测定乙醛酸和乙二醛[J].分析化学,1997,25(9):1086-1089.
【4】朱明华.仪器分析.第三版[M].北京:高等教育出版社,2000:13-90.
【5】刘庆彬,薄海静,郭秀斌,等.乙醛酸水溶液的高效液相色谱分析[J].分析化学,1998,26(1):119.
【6】施奈德 L R,格莱吉客 J L,柯克兰 J J.实用高效液相色谱法的建立[M].北京:科学出版社,1998:85-86.
【7】Bevilacqua A E,Califano A N.Determination of organic acids in dairy products by high performance liquid chromatograghy[J].Food Science,1989,54(4):1076-1077.
相关信息