双水相体系萃取分离-紫外分光光度法测定桑叶中芦
UV SPECTROPHOTOMETRIC DETERMINATION OF RUTIN IN MULBERRY LEAVES BY THE BINARY AQUEOUS-PHASE EXTRACTION
摘 要
通过试验选择聚乙二醇(PEG 1000)与聚乙烯吡咯烷酮(PVP 30000)为组合表面活性剂,(NH4)2SO4-H2O为双水相体系.在VPEG 1000/VPVP 30000=2.00 mL/2.00 mL,(NH4)2SO4用量为1.5 g,pH 6.0的B.R缓冲溶液最佳萃取条件下,经两次萃取,桑叶中芦丁定量进入PEG-PVP相.用紫外分光光度法在375 nm波长处直接萃取测定桑叶提取液中的芦丁含量.试验结果表明,方法的相对标准偏差≤3.8%(n=7),样品加入回收率为96.5%.试验表明,方法具有良好的选择性.此外,用试样超声提取率较常规索氏提取率高.
紫外分光光度法
芦丁
桑叶
Binary aqueous-phase extraction system
UV-spectrophotometry
Rutin
Mulberry leaves
Abstract
Composite surfactants of PEG1000 and PVP30000 and definite amounts of (NH4)2SO4 and H2O were chosen experimentally as components of the binary aqueousphase extraction system.The volume ratio between PEG 1000 and PVP 30000,the acidity of the extraction solution and the amount of (NH4)2SO4 used,which are the influential factors to the phase separation,were studied and the optimized conditions found were as follows:① VPEG 1000/VPVP 30000=2.00 mL/2.00 mL;② acidity:pH 6.0,using B.R.buffer;③ amount of (NH4)2SO4 added:1.5 g.Rutin in mulberry leaves was extracted with the binary aqueous-phase extraction system (BAPES) quantitatively into the PEG 10000-PVP 30000 phase by two extractions and determined UV-spectrophotometrically in that phase at 375 nm.Values of RSD and recovery found were ≤3.8% and 96.5% respectively.Results of interference test showed that the selectivity of this method was also acceptable.The use of ultrasonic wave for shaking of the extraction mixture showed higher efficiency than the conventional soxhlet method.
中图分类号 O657.37
所属栏目 工作简报
基金项目 江西省自然科学基金(No.0320021)
收稿日期 2004/11/12
修改稿日期
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备注练萍(1965-),女,江西赣州人,副教授,从事分析及分析化学教学与研究工作.
引用该论文: LIAN Ping,LI Lei,LAI Wen-lin,XUE Jun. UV SPECTROPHOTOMETRIC DETERMINATION OF RUTIN IN MULBERRY LEAVES BY THE BINARY AQUEOUS-PHASE EXTRACTION[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(10): 821～823
丁练萍,李蕾,赖闻玲,薛珺. 双水相体系萃取分离-紫外分光光度法测定桑叶中芦[J]. 理化检验－化学分册, 2006, 42(10): 821～823
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参考文献
【1】Middleton E J R,Kandashwami C,Cheoharides T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacol Rev,2000,52:673-751．
【2】练萍,李蕾,戚琦,等.PEG 600-Triton X-100组合表面活性剂双水相体系萃取测定类黄酮[J].分析试验室,2005,24(5):55-58.
【3】乙引,刘宁.测定植物槲皮素含量的高效液相色谱法[J].植物生理学通讯,2000,36(5):445-446.
【4】孙莲,孟磊,陈坚.毛细管电泳法测定桑叶中的黄酮类成分——芦丁和槲皮素[J].色谱,2001,19(5):395-397．
【5】Li Lei,Liu Feng,Xiang Xu-kong,et al.Investigation of a liquid-liquid extraction system based on non-ionic surfactant-salt H2O and mechanism of drug extraction[J].Anal Chim Acta,2002,452:321-328．
【6】李蕾,薛君,练萍.组合表面活性剂-盐-水双水相体系萃取水杨酸和洛美沙星[J].分析化学,2004,32(1):96-98．
【7】练萍,李蕾,薛君.PEG-PVP组合表面活性剂-(NH4)2SO4-H2O双水相体系萃取Eu3+的研究[J].稀土,2003,24(3):71-74．
【2】练萍,李蕾,戚琦,等.PEG 600-Triton X-100组合表面活性剂双水相体系萃取测定类黄酮[J].分析试验室,2005,24(5):55-58.
【3】乙引,刘宁.测定植物槲皮素含量的高效液相色谱法[J].植物生理学通讯,2000,36(5):445-446.
【4】孙莲,孟磊,陈坚.毛细管电泳法测定桑叶中的黄酮类成分——芦丁和槲皮素[J].色谱,2001,19(5):395-397．
【5】Li Lei,Liu Feng,Xiang Xu-kong,et al.Investigation of a liquid-liquid extraction system based on non-ionic surfactant-salt H2O and mechanism of drug extraction[J].Anal Chim Acta,2002,452:321-328．
【6】李蕾,薛君,练萍.组合表面活性剂-盐-水双水相体系萃取水杨酸和洛美沙星[J].分析化学,2004,32(1):96-98．
【7】练萍,李蕾,薛君.PEG-PVP组合表面活性剂-(NH4)2SO4-H2O双水相体系萃取Eu3+的研究[J].稀土,2003,24(3):71-74．
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