HYPHENATED GC-MS DETERMINATION OF SMELLY SUBSTANCES IN EUTROPHIC WATER
摘 要
通过固相微萃取富集,并采用气相色谱-质谱联用(GC-MS)测定了水中常见的两种异味化合物,即2-甲基异茨醇(2-MIB)、土味素(GSM),并对富营养化水体中的挥发性物质进行了初步分析.不同的异味物质吸附于75 μm Carboxen/PDMS纤维涂层处理后,通过DB-WAX毛细管色谱柱得以分离.采用选择离子监测模式(SIM)对两种化合物(2-MIB m/z=95,GSM m/z=112)进行外标法定量分析,从而提高检测灵敏度.线性范围2-MIB为0.5~500 ng·L-1、GSM为1.0~500 ng·L-1,检出限分别为 0.1,0.4 ng·L-1.用该法对富营养化水源水监测,2-MIB及GSM的分析结果的RSD值及加标回收率为3.35%,7.68%和105.0%,88.3%.
Abstract
Hyphenated GC-MS was applied to the determination of two smelly substances,namely 2-methyl isoborneol (2-MIB) and geosmin (GSM),commonly found in eutrophic water,after their separation by solid-phase microextraction (SPME).A preliminary analysis of volatile substances in the eutrophic water was also done.Different kinds of smelly substances were adsorbed on 75 μm carboxen/PDMS fiber and separated by passing through the DB-WAX capillary chromatographic column (30 mm×0.25 mm,0.25 μm).The above mentioned smelly substances,2-MIB and GSM,were determined by GC-MS with selected ion monitoring mode,using m/z=95 for 2-MIB and m/z=112 for GSM.The sensitivity of the method was improved and linear relationship between peak area and concentration of the smelly substances were obtained in the ranges of 0.5-500 ng·L-1 (for 2-MIB) and 1.0-500 ng·L-1 (for GSM),with detection limits of 0.1 ng·L-1 and 0.4 ng·L-1 respectively.In its application to the analysis of water samples taken from the surface layer of Taihu Lake,values of RSD of the analytical results (n=8) of 3.35% and 7.68% and average recovery of 105.0% and 88.3% were obtained for 2-MIB and GSM respectively.
中图分类号 O657.7
所属栏目 工作简报
基金项目 农业部海洋与河口渔业重点开放实验室开放课题(开-2-04-13),江南大学青年科学基金(2004LQN014)
收稿日期 2006/2/22
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备注王利平(1965-),男,江苏无锡人,高级工程师,主要从事食品安全检验及食品风味研究工作.
引用该论文: WANG Li-ping,YU Rui-peng,LIU Yang-min,GONG Xiao-qing,WU Sheng-fang. HYPHENATED GC-MS DETERMINATION OF SMELLY SUBSTANCES IN EUTROPHIC WATER[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2006, 42(12): 1013~1015
王利平,虞锐鹏,刘扬岷,贡小清,吴胜芳. 气相色谱-质谱联用测定富营养化水体中的异味物质[J]. 理化检验-化学分册, 2006, 42(12): 1013~1015
被引情况:
【1】闫晖敏,顾海东,许文雅,张丽君,张磊,张占恩, "分散固相萃取-气相色谱-质谱法测定地表水中的异味物质",理化检验-化学分册 51, 82-85(2015)
【2】李祖光,朱国华,曹慧,莫卫民, "顶空固相微萃取-气相色谱-质谱法测定二苯甲酮类防晒剂中残留溶剂",理化检验-化学分册 45, 1422-1423(2009)
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