薄层层析法分离菊芋汁中低聚糖
Separation of Oligosaccharides in Juice of Jerusalem Artichoke by Thin Layer Chromatography
摘 要
采用薄层层析法(TLC)分离菊芋汁中的低聚糖成分.固定相为硅胶板GF254,展层剂为V正丁醇∶V异丙醇∶V乙酸∶V水=7∶5∶2∶4,显色剂为苯胺(4 mL)-二苯胺(4 g)-磷酸(20 mL)(溶于200 mL丙酮中)试剂.该展层体系可用于快速层析分离菊芋汁中的混合低聚糖,经过两次展层可达到良好分离结果.
菊芋
低聚糖
Thin layer chromatography
Jerusalem Artichoke
Oligosaccharide
Abstract
Thin layer chromatography (TLC) was applied to the separation of oligosaccharides in juice of Jerusalem Artichoke.The silica gel plate (GF 254) was used as the stationary phase,and a mixed solvent,containing 1-butanol,2-propanol,acetic acid and H2O in the ratio of 7∶5∶2∶4,was used as the developing reagent.The colouring reagent was a mixed solution containing aniline (4 mL),diphenylamine (4 g) and conc.H3PO4 (20 mL) dissolved in 200 mL of acetone.Satisfactory separation of various kinds of oligosaccharides in juice of Jerusalem Artichoke was attained by a double development with the mixed solvent.The method proposed was feasible to be used for rapid TLC separation.
中图分类号 O657.37
所属栏目 工作简报
基金项目 863计划(2001AA246041和2004AA246040);国家自然科学基金(39970549);948计划(2001-244);农业部农业科技成果转化示范项目(农业部农计函(2003)165号).
收稿日期 2005/9/15
修改稿日期
网络出版日期
作者单位点击查看
备注杨雅麟(1975-),女,贵州人,助研,博士研究生,研究方向为生物技术.
引用该论文: YANG Ya-lin,WANG Jian-hua,ZHANG Fan,TENG Da. Separation of Oligosaccharides in Juice of Jerusalem Artichoke by Thin Layer Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(3): 229～230
杨雅麟,王建华,张帆,滕达. 薄层层析法分离菊芋汁中低聚糖[J]. 理化检验－化学分册, 2007, 43(3): 229～230
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】Yokota A,Enomoto K,Tomita F.Purification and properties of an inulin fructotransferase (depolymerizing) by Arthrobacter sp.H65-7[J].J Ferment Bioeng,1991,72(4):262-265．
【2】张龙翔,张庭芳,李令媛.生化实验方法和技术[M].北京:人民教育出版社,1982:15．
【3】张桂,李俊英,陈学武,等.薄层层析法测定纤维素材料水解液中混合糖的研究[J].理化检验-化学分册,2002,38(2):81-84．
【4】Yokota A,Hirayama S,Enomoto K,et al.Production of inulin fructotransferase (depolymerizing) by Arthrobacter sp.H65-7 and preparation of DFA Ⅲ from inulin by the enzyme[J].J Ferment Bioeng,1991,72(4):258-261．
【2】张龙翔,张庭芳,李令媛.生化实验方法和技术[M].北京:人民教育出版社,1982:15．
【3】张桂,李俊英,陈学武,等.薄层层析法测定纤维素材料水解液中混合糖的研究[J].理化检验-化学分册,2002,38(2):81-84．
【4】Yokota A,Hirayama S,Enomoto K,et al.Production of inulin fructotransferase (depolymerizing) by Arthrobacter sp.H65-7 and preparation of DFA Ⅲ from inulin by the enzyme[J].J Ferment Bioeng,1991,72(4):258-261．
相关信息
