在蛹虫草中腺苷和虫草素含量的反相高效液相色谱分析中,所用色谱柱为Waters NOVA-PAK C18(3.9 mm×300 mm,4 μm),流动相为0.01 mol·L-1 KH2PO4-K2HPO4缓冲溶液(pH 6.86)+1%(体积分数)四氢呋喃,流速为1.0 mL·min-1.紫外检测的波长为260 nm,峰面积与腺苷浓度和虫草素浓度之间的线性范围依次为1.27~50.5 mg·L-1和3.23~129.0 mg·L-1,其检出限依次为0.18 ng和0.25 ng.在腺苷及虫草素的浓度水平依次为5.05 mg·L-1和12.9 mg·L-1时测试方法的精密度,求算得RSD(n=7)值依次为0.094%和0.792%.用标准加入法试验方法的回收率,求得两者的平均回收率依次为103.2%和98.5%.

In the RP-HPLC determination of adenosine and cordycepin in cordyceps,Waters NOVA-PAK C18 (3.9 mm×300 mm,4 μm) was used as chromatographic column,and KH2PO4-K2HPO4 buffer of 0.01 mol·L-1 (pH 6.86) +1%(V/V) tetrahydrofuran was used as mobile phase,passing through the column at the flow-rate of 1.0 mL·min-1.UV-detection at wavelength of 260 nm was used in the determinations.Linear relationships between the peak area and concentration of adenosine and cordycepin were obtained in the ranges of 1.27-50.5 mg·L-1 and 3.23-129.0 mg·L-1 respectively.Detection limit of adenosine was found to be 0.18 ng and of cordycepin to be 0.25 ng.Precision of the method was tested at the concentration levels of 5.05 mg·L-1 (for adenosine) and 12.9 mg·L-1 (for cordycepin),and the values of RSD′s (n=7) found were 0.094% and 0.792% respectively.Recovery was tested by the standard addition method,and values of average recovery obtained were 103.2% (for adenosine) and 98.5% (for cordycepin).