Rapid Determination of Flavonoid in Saururus Chinesis (Lour) Baill by High Performance Liquid Chromatography
摘 要
研究了用固相萃取预分离,高效液相色谱法测定三白草中黄酮类物质.三白草中的黄酮用甲醇(90+10)加热回流提取,提取液用Waters Sep-Pak-C18固相萃取小柱预分离,以ZORBAX Stable Bound (4.6 mm×50 mm,1.8 μm)色谱柱为固定相,磷酸(2+998)和甲醇(以体积比50比50)为流动相,在该色谱条件下,三白草中主要的黄酮成分在1.5 min内可达到基线分离;用紫外二极管矩阵检测器检测.用该方法测定了5种三白草样品中的黄酮类物质,回收率在97%~103%之间,相对标准偏差在1.6%~2.2%之间.
Abstract
The sample was pretreated by refluxing with CH3OH+H2O(90+10) for 45 min and extracting on Sep-Pak-C18 microcolumn.In the HPLC analysis,the Zorbax stable Bound (4.6 mm×50 mm,1.8 μm) was used as stationary phase,and a mixture of H3PO4 (2+998) and methanol with a ratio of 1 to 1 was used as the mobile phase.Main flavones in the sample were separated from the base-line within 1.5 min.UV-diode array detector was used in the determination at the wavelength of 360 nm.Samples of 5 kinds of saururus chinesis (Lour) baill was analyzed by the proposed method,giving values of recovery in the range 97%-103%,and RSD′s (n=5) in the range 1.6%-2.2%.
中图分类号 O657.37
所属栏目 工作简报
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收稿日期 2005/12/13
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备注彭永芳(1964-),女,云南昆明人,教授,硕士,从事化学教学研究工作.
引用该论文: PENG Yong-fang,MA Yin-hai,GUO Ya-dong,YANG Guang-yu,YIN Jia-yuan. Rapid Determination of Flavonoid in Saururus Chinesis (Lour) Baill by High Performance Liquid Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(6): 463~464
彭永芳,马银海,郭亚东,杨光宇,尹家元. 高效液相色谱法快速测定三白草中黄酮类物质[J]. 理化检验-化学分册, 2007, 43(6): 463~464
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参考文献
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【5】许雪琴,叶蔚芝,齐秀贞,等.反相高效液相色谱法测定三白草中椒皮素和芦丁的含量[J].福州大学学报:自然科学版,2002,30(6):870-872.
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